Recombinant Arabidopsis thaliana NAC domain-containing protein 43 (NAC043)

Code CSB-YP768026DOA
MSDS
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Source Yeast
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Code CSB-EP768026DOA
MSDS
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Source E.coli
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Code CSB-EP768026DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP768026DOA
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Source Baculovirus
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Code CSB-MP768026DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NAC043
Uniprot No.
Alternative Names
NAC043; EMB2301; NST1; At2g46770; F19D11.5; NAC domain-containing protein 43; ANAC043; Protein EMBRYO DEFECTIVE 2301; Protein NAC SECONDARY WALL THICKENING PROMOTING FACTOR 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-365
Target Protein Sequence
MMSKSMSISV NGQSQVPPGF RFHPTEEELL QYYLRKKVNS IEIDLDVIRD VDLNKLEPWD IQEMCKIGTT PQNDWYFFSH KDKKYPTGTR TNRATAAGFW KATGRDKIIY SNGRRIGMRK TLVFYKGRAP HGQKSDWIMH EYRLDDNIIS PEDVTVHEVV SIIGEASQDE GWVVCRIFKK KNLHKTLNSP VGGASLSGGG DTPKTTSSQI FNEDTLDQFL ELMGRSCKEE LNLDPFMKLP NLESPNSQAI NNCHVSSPDT NHNIHVSNVV DTSFVTSWAA LDRLVASQLN GPTSYSITAV NESHVGHDHL ALPSVRSPYP SLNRSASYHA GLTQEYTPEM ELWNTTTSSL SSSPGPFCHV SNGSG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription activator of genes involved in biosynthesis of secondary walls. Together with NST2 and NST3, required for the secondary cell wall thickening of sclerenchymatous fibers, secondary xylem (tracheary elements), and of the anther endocethium, which is necessary for anther dehiscence. May also regulate the secondary cell wall lignification of other tissues.
Gene References into Functions
  1. The MYB26 protein is localized specifically to the anther endothecium nuclei and that it directly regulates two NAC domain genes, NST1 and NST2, which are critical for the induction of secondary thickening biosynthesis genes. PMID: 28724622
  2. These results demonstrate that NST2 together with SND1 and NST1 regulate secondary wall biosynthesis in fibers of stems. PMID: 25751728
  3. NST1 and NST3 redundantly regulate the secondary wall thickenings in interfascicular fiber of inflorescence stems and secondary xylem of hypocotyls in Arabidopsis without affecting the formation of cells destined to be woody tissues. [NST1] PMID: 17237351
  4. First line evidence demonstrating that SND1 and NST1 function redundantly in the regulation of secondary wall synthesis in fibers is presented. PMID: 17333250
  5. NST1 and NST3 regulate the formation of secondary walls in siliques which regulates pod shattering. PMID: 18657234

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in various aboveground tissues undergoing thickening of the lignified secondary wall such as anthers, filaments of stamens, the base of carpels, styles, the boundaries between siliques and pedicels, the midrib of leaf veins, and inflorescence st
Database Links

KEGG: ath:AT2G46770

STRING: 3702.AT2G46770.1

UniGene: At.36457

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