Recombinant Arabidopsis thaliana NAC domain-containing protein 78 (NAC078), partial

Code CSB-YP769552DOA
MSDS
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Source Yeast
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Code CSB-EP769552DOA
MSDS
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Source E.coli
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Code CSB-EP769552DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP769552DOA
MSDS
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Source Baculovirus
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Code CSB-MP769552DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NAC078
Uniprot No.
Alternative Names
NAC078; NAC2; NTL11; At5g04410; T32M21.10; NAC domain-containing protein 78; ANAC078; Protein NTM1-like 11
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP). Transcripition activator associated with the induction of genes related to flavonoid biosynthesis and required for the accumulation of anthocyanins in response to high light stress. Plays a role in the regulation of 20S and 26S proteasomes in response to high light stress.
Gene References into Functions
  1. PI4K gamma 5 is important for the normal function of ANAC078 and PI4K gamma 5 is required for ANAC078 cleavage and proteolytic activation [ANAC078] PMID: 27529511
  2. RPX is a negative regulator of leaf organ size. RPX binds to the promoter of genes encoding 26S proteasomal proteins in vivo, resulting in increased gene expression and proteasome activity. PMID: 23252408
  3. The transcript levels of 13 proteasome subunit genes, the protein levels of proteasomes, and the activities of the 26S proteasome in ANAC078-overexpressing Arabidopsis plants (Ox-ANAC078) and knockout ANAC078 (KO-ANAC078) mutants, was investigated. PMID: 21889048
  4. ANAC092 strongly responds to long-term salt stress that triggers senescence. PMID: 20113437
  5. Molecular mechanisms underlying the cold temperature regulation of flowering time of Arabidopsis are controled by NAC2. PMID: 17653269
  6. ANAC078 protein functions as a transcription factor, and is associated with expression of genes related to flavonoid biosynthesis, leading to the accumulation of anthocyanins, in response to high light stress. PMID: 19887540

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Subcellular Location
Membrane; Single-pass membrane protein. Nucleus.
Tissue Specificity
Expressed in root meristem (Ref.1). Expressed in roots, rosette leaves, cauline leaves, shoot apex, stems and flowers.
Database Links

KEGG: ath:AT5G04410

STRING: 3702.AT5G04410.1

UniGene: At.22852

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