Recombinant Arabidopsis thaliana Nitrile-specifier protein 1 (NSP1)

Code CSB-YP886656DOA
MSDS
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Source Yeast
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Code CSB-EP886656DOA
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Source E.coli
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Code CSB-EP886656DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP886656DOA
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Source Baculovirus
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Code CSB-MP886656DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NSP1
Uniprot No.
Alternative Names
NSP1; JAL28; At3g16400; MDC8.2; T02O04.11; Nitrile-specifier protein 1; AtNSP1; Jacalin-related lectin 28
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-470
Target Protein Sequence
MAQKLEAKGG EMGDVWDDGV YENVRKVYVG QAQYGIAFVK FEYVNGSQVV VGDEHGKKTE LGVEEFEIDA DDYIVYVEGY REKVNDMTSE MITFLSIKTF KGKTSHPIEK RPGVKFVLHG GKIVGFHGRS TDVLHSLGAY VSLSSTIKLL GKWIKVEQKG EGPGLRCSHG IAQVGNKIYS FGGEFTPNQP IDKHLYVFDL ETRTWSISPA TGDVPHLSCL GVRMVSVGST LYVFGGRDAS RQYNGFYSFD TTTNEWKLLT PVEEGPTPRS FHSMAADEEN VYVFGGVSAT ARLNTLDSYN IVDKKWFHCS TPGDSLTARG GAGLEVVQGK VWVVYGFNGC EVDDVHYYDP VQDKWTQVET FGVRPSERSV FASAAIGKHI VIFGGEIAMD PLAHVGPGQL TDGTFALDTE TLQWERLDKF GGEEETPSSR GWTASTTATI DGKKGLVMHG GKAPTNDRFD DLFFYGIDSA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Responsible for constitutive and herbivore-induced simple nitrile formation. Promotes simple nitriles, but not epithionitrile or thiocyanate formation. Converts allylglucosinolate, 4-methylsulfinylbutylglucosinolate, 4-methylthiobutylglucosinolate and benzylglucosinolate to their corresponding simple nitriles in the presence of myrosinase.
Gene References into Functions
  1. Crystal structure of the nitrile-specifier protein NSP1 PMID: 28479247
  2. At3g16400 encodes a nitrile-specifier protein that controls constitutive and herbivore-induced simple nitrile formation in rosette leaves. PMID: 18987211
  3. AtNSP1 is responsible for constitutive and herbivore-induced simple nitrile formation in Col-0 rosette leaves but do not support epithionitrile formation. [AtNSP1] PMID: 18987211
Protein Families
Jacalin lectin family
Database Links

KEGG: ath:AT3G16400

STRING: 3702.AT3G16400.1

UniGene: At.22512

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