Recombinant Arabidopsis thaliana Non-symbiotic hemoglobin 1 (AHB1)

Code CSB-YP515841DOA
MSDS
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Source Yeast
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Code CSB-EP515841DOA
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Source E.coli
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Code CSB-EP515841DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP515841DOA
MSDS
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Source Baculovirus
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Code CSB-MP515841DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AHB1
Uniprot No.
Alternative Names
AHB1; GLB1; At2g16060; F7H1.8Non-symbiotic hemoglobin 1; ARAth GLB1; Hb1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-160
Target Protein Sequence
MESEGKIVFT EEQEALVVKS WSVMKKNSAE LGLKLFIKIF EIAPTTKKMF SFLRDSPIPA EQNPKLKPHA MSVFVMCCES AVQLRKTGKV TVRETTLKRL GASHSKYGVV DEHFEVAKYA LLETIKEAVP EMWSPEMKVA WGQAYDHLVA AIKAEMNLSN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May not function as an oxygen storage or transport protein, but might act as an oxygen sensor or play a role in electron transfer, possibly to a bound oxygen molecule. Has an unusually high affinity for O(2) because of a very low dissociation constant.
Gene References into Functions
  1. Findings provide evidence supporting the functional implications of the conformational rearrangement found for the distal histidine in AHb1, which mimics the gating role proposed for the same residue in myoglobin. PMID: 21428382
  2. AtHb1 overexpression led to several alterations in transcriptional and metabolic networks, resulting in improved seed yield. PMID: 21406103
  3. Rebinding of NO ligands was observed only from site B of AHb1, regardless of conformation. PMID: 20666470
  4. the nature and distribution of internal cavities in the protein matrix PMID: 19954242
  5. The structures of AHb1 and AHb2, modeled on the basis of the homologous rice hemoglobin, exhibit a different cavity system that is fully compatible with the observed ligand binding kinetics PMID: 17298064
  6. Finding strongly supports the idea that AHb1 and AHb2 have distinct physiological functions. PMID: 17555890

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Protein Families
Plant globin family
Tissue Specificity
Expressed in roots and rosette leaves.
Database Links

KEGG: ath:AT2G16060

STRING: 3702.AT2G16060.1

UniGene: At.40321

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