Recombinant Arabidopsis thaliana Nudix hydrolase 19, chloroplastic (NUDT19)

Code CSB-YP846406DOA
MSDS
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Source Yeast
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Code CSB-EP846406DOA
MSDS
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Source E.coli
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Code CSB-EP846406DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP846406DOA
MSDS
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Source Baculovirus
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Code CSB-MP846406DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NUDT19
Uniprot No.
Alternative Names
NUDT19; NUDX19; At5g20070; F28I16.220; Nudix hydrolase 19; chloroplastic; AtNUDT19; EC 3.6.1.22; NADH pyrophosphatase NUDT19
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
37-438
Target Protein Sequence
SMNL KTHAFAGNPL KSKTPKSTDP FSPTSAFESL KTLIPVIPNH STPSPDFKVL PFSKGRPLVF SSGGDANTTP IWHLGWVSLA DCKVLLASCG VDLNEDSLVY LGPKLEEDLV YWAVDLAEDG FVSELGGRKL CFVELRTLMV AADWADQRAM DELAIAGNAR ALLEWHNVSQ FCGSCGSKTF PKEAGRRKQC SDETCRKRVY PRVDPVVIML VIDRENDRAL LSRQSRYVPR MWSCLAGFIE PGESLEEAVR RETWEETGIE VGDVVYHSSQ PWPVGPSSMP CQLMLGFFAF AKTLDINVDK EELEDAQWHS REEVKKALAV AEYRKAQRTA AAKVEQICKG VERSQSLSTD FNLESGELAP MFIPGPFAIA HHLISAWVNQ APDDVHSKQQ AGVSLSSL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA. The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Mediates the hydrolysis of some nucleoside diphosphate derivatives. Has a high affinity for NADPH compared with that for NADH.
Gene References into Functions
  1. ANUDX19 acts as an NADPH pyrophosphohydrolase to modulate cellular levels and redox states of pyridine nucleotides and fine-tunes photooxidative stress response. PMID: 27874073
  2. The results suggest that AtNUDX19 could be a key factor in modulating the NADPH pool in plants and consequently in redox homeostasis. PMID: 26878367
Subcellular Location
Plastid, chloroplast.
Protein Families
Nudix hydrolase family, NudC subfamily
Tissue Specificity
Expressed in roots, leaves, stems and inflorescences.
Database Links

KEGG: ath:AT5G20070

STRING: 3702.AT5G20070.1

UniGene: At.19787

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