Recombinant Arabidopsis thaliana Pathogenesis-related protein 1 (At2g14610)

Code CSB-YP339325DOA
MSDS
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Source Yeast
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Code CSB-EP339325DOA
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Source E.coli
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Code CSB-EP339325DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339325DOA
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Source Baculovirus
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Code CSB-MP339325DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
At2g14610
Uniprot No.
Alternative Names
At2g14610; T6B13.15; Pathogenesis-related protein 1; PR-1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
27-161
Target Protein Sequence
QDSP QDYLRVHNQA RGAVGVGPMQ WDERVAAYAR SYAEQLRGNC RLIHSGGPYG ENLAWGSGDL SGVSAVNMWV SEKANYNYAA NTCNGVCGHY TQVVWRKSVR LGCAKVRCNN GGTIISCNYD PRGNYVNEKP Y
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Partially responsible for acquired pathogen resistance.
Gene References into Functions
  1. CRWN1 represses PR1 transcription by interacting with NTL9 PMID: 28943325
  2. We also demonstrated that LPS-treated atlbr mutant Arabidopsis exhibit a significant delay of induction of defence-related gene pathogenesis-related 1 (PR1) but no other PR genes. Furthermore, LPS-treated atlbr mutants showed defects in reactive oxygen species (ROS) generation PMID: 27273538
  3. Resistance induced by Paenibacillus alvei K165 depends on salicylate and jasmonate-dependent defense pathways, as evidenced by an increased transient accumulation of PR1 and PDF1.2 transcripts in the aerial parts of infected plants treated with K165. PMID: 26780421
  4. Di19, a new type of transcription factor, directly up-regulates the expressions of PR1, PR2, and PR5 in response to drought stress. PMID: 23404561
  5. Dtta indicate that the expression of marker genes PR1 and BGL2 for the salicylic acid (SA) and the PDF1.2 for the jasmonic acid-ethylene (JA-ET) signalling pathways was enhanced in 10 muM Cd-treated non-infected plants. PMID: 23070523
  6. It is reported that salicylic acid (SA)-treated tir1 afb2 mutant shows enhanced transcript level of a pathogenesis related gene, PR1. PMID: 21358272
  7. Natural variation in salicylic acid-induced PR-1 expression is associated with basal resistance against the hemi-biotrophic pathogen Pst DC3000. PMID: 21414016
  8. In the absence of LS5 and LS7, NPR1 activates the PR-1 promoter through a mechanism that requires LS4 PMID: 20935179
  9. Expression in comparison to At2g19990; recommendation to change the annotation. PMID: 15293050
  10. Lipooligosaccharide and the lipid A and core oligosaccharides derived from it were all able to induce the defense-related gene PR1 in Arabidopsis and to prevent the hypersensitive response caused by avirulent bacteria. PMID: 16048996
  11. The transcript level of PR1, and PR2 were increased in ascorbate-deficient mutants. PMID: 18849295

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Subcellular Location
Secreted, extracellular space, apoplast.
Protein Families
CRISP family
Database Links

KEGG: ath:AT2G14610

STRING: 3702.AT2G14610.1

UniGene: At.321

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