Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At2g31400, chloroplastic (At2g31400), partial

Code CSB-YP890347DOA
MSDS
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Source Yeast
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Code CSB-EP890347DOA
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Source E.coli
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Code CSB-EP890347DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890347DOA
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Source Baculovirus
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Code CSB-MP890347DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
At2g31400
Uniprot No.
Alternative Names
At2g31400; T28P16.11; Pentatricopeptide repeat-containing protein At2g31400; chloroplastic
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Gene References into Functions
  1. RH50 and GUN1 are functionally related and that this function is associated with plastid gene expression, in particular ribosome functioning. PMID: 29138350
  2. Defects in GUN1 or mTERF4 de-repressed the expression of specific plastid mRNAs in the presence of lincomycin (LIN). In wild-type plants, treatment with LIN or spectinomycin (SPE) inhibited processing of plastid transcripts. PMID: 26685190
  3. Data show that the the genomes uncoupled 1 (gun1) mutation genetically interacts with mutations of genes for the chloroplast ribosomal proteins S1 (PRPS1). PMID: 26823545
  4. GUN1 mediated plastid sugar metabolism is involved in nuclear gene expression regulation. PMID: 25628228
  5. GUN1 and GUN4 partially restored the expression patterns of the previously detected chloroplast-associated genes, thereby ameliorating the slow-greening phenotype of sg1. PMID: 24420572
  6. The findings indicate a central role for GUN1 in plastid, sucrose and ABA signalling in early seedling development. PMID: 21673514
  7. It was found that plastid signals that depend on GUN1 can affect cotyledon opening and expansion, anthocyanin biosynthesis, and hypocotyl elongation. [GENOMES UNCOUPLED 1] [GUN1] PMID: 19140931
  8. GUN1 (genomes uncoupled1)is involved in the regulation of seedling development and anthocyanin biosynthesis, and indicate a complex interplay between sucrose and plastid signalling pathways. [GUN1] PMID: 20605896
  9. results show GUN1 & ABI4 are common to all 3 plastid-to-nucleus retrograde signaling pathways; model proposed in which indicators of aberrant plastid function are integrated upstream of GUN1 in plastids leading to ABI4-mediated repression of nuclear genes PMID: 17395793

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Subcellular Location
Plastid, chloroplast.
Protein Families
PPR family, P subfamily
Database Links

KEGG: ath:AT2G31400

STRING: 3702.AT2G31400.1

UniGene: At.20815

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