Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g60960, mitochondrial (At5g60960)

Code CSB-YP863813DOA
MSDS
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Source Yeast
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Code CSB-EP863813DOA
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Source E.coli
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Code CSB-EP863813DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863813DOA
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Source Baculovirus
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Code CSB-MP863813DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PNM1
Uniprot No.
Alternative Names
PNM1; At5g60960; MSL3.8; Pentatricopeptide repeat-containing protein PNM1; mitochondrial; PPR PROTEIN LOCALIZED TO THE NUCLEUS AND MITOCHONDRIA 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
61-521
Target Protein Sequence
SETNAESESL DSNEIALSFS KELTGNPDAE SQTISQRFNL SFSHITPNPD LILQTLNLSP EAGRAALGFN EWLDSNSNFS HTDETVSFFV DYFGRRKDFK GMLEIISKYK GIAGGKTLES AIDRLVRAGR PKQVTDFFEK MENDYGLKRD KESLTLVVKK LCEKGHASIA EKMVKNTANE IFPDENICDL LISGWCIAEK LDEATRLAGE MSRGGFEIGT KAYNMMLDCV CKLCRKKDPF KLQPEVEKVL LEMEFRGVPR NTETFNVLIN NLCKIRRTEE AMTLFGRMGE WGCQPDAETY LVLIRSLYQA ARIGEGDEMI DKMKSAGYGE LLNKKEYYGF LKILCGIERL EHAMSVFKSM KANGCKPGIK TYDLLMGKMC ANNQLTRANG LYKEAAKKGI AVSPKEYRVD PRFMKKKTKE VDSNVKKRET LPEKTARKKK RLKQINMSFV KKPHNKMRRR M
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding protein that functions in both mitochondrion and nucleus. In mitochondrion, it is associated with polysomes and may play a role in translation. Required during embryogenesis. In nucleus, might be involved in the regulation of its own gene expression.
Gene References into Functions
  1. PNM1 interacts with the nucleosome assembly protein NAP1 and the nuclear transcription factor TCP8 in the nucleus of plant cells, and is a potential coordinator of the expression of the nuclear and mitochondrial genomes. PMID: 21297037
Subcellular Location
Mitochondrion matrix. Nucleus. Note=Can localize to both mitochondrion and nucleus.
Protein Families
PPR family, P subfamily
Tissue Specificity
Expressed in root tips, lateral root primordia and leaf primordia. Highly detected in the mature pollen grains.
Database Links

KEGG: ath:AT5G60960

STRING: 3702.AT5G60960.1

UniGene: At.8467

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