Recombinant Arabidopsis thaliana Peptidyl-prolyl cis-trans isomerase CYP40 (CYP40)

Code CSB-YP861222DOA
MSDS
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Source Yeast
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Code CSB-EP861222DOA
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Source E.coli
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Code CSB-EP861222DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861222DOA
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Source Baculovirus
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Code CSB-MP861222DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP40
Uniprot No.
Alternative Names
CYP40; SQN; At2g15790; F19G14.21Peptidyl-prolyl cis-trans isomerase CYP40; PPIase CYP40; EC 5.2.1.8; Cyclophilin of 40 kDa; Cyclophilin-40; Protein SQUINT; Rotamase CYP40
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-361
Target Protein Sequence
MGRSKCFMDI SIGGELEGRI VIELYDDVVP KTAENFRLLC TGEKGLGPNT GVPLHYKGNR FHRVIKGFMI QGGDISANDG TGGESIYGLK FDDENFELKH ERKGMLSMAN SGPNTNGSQF FITTTRTSHL DGKHVVFGRV TKGMGVVRSI EHVSIEEQSC PSQDVVIHDC GEIPEGADDG ICDFFKDGDV YPDWPIDLNE SPAELSWWME TVDFVKAHGN EHFKKQDYKM ALRKYRKALR YLDICWEKEG IDEETSTALR KTKSQIFTNS AACKLKFGDA KGALLDTEFA MRDEDNNVKA LFRQGQAYMA LNNVDAAAES LEKALQFEPN DAGIKKEYAA VMKKIAFRDN EEKKQYRKMF V
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in promoting the expression of the juvenile phase of vegetative development, and, to a lower extent, in regulating the positioning of floral buds, floral morphogenesis and the expression of HSPs.
Gene References into Functions
  1. One of the targets of miR172, APETALA2 (AP2), antagonizes CLV signalling. The ap2-2 mutation strongly suppresses sqn meristem phenotypes, indicating that the effect of SQN on stem cell dynamics is largely, but not fully, mediated by the miR172/AP2 tandem. PMID: 26269626
  2. SQN shows variable embryonic expression levels between accessions and altered ZLL-dependency in transgenic assays, confirming a key role in stem cell maintenance. PMID: 24252363
  3. the interaction between CyP40 and Hsp90 is conserved in plants and this interaction is essential for the function of CyP40 PMID: 21908611
  4. genetic data suggest that RBL, SQN, and, to a lesser extent, ULT1 also influence SUPERMAN activity PMID: 18441215
Subcellular Location
Cytoplasm.
Protein Families
Cyclophilin-type PPIase family
Tissue Specificity
Expressed at low levels in seedlings, leaves and flowers.
Database Links

KEGG: ath:AT2G15790

STRING: 3702.AT2G15790.1

UniGene: At.13863

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