Recombinant Arabidopsis thaliana Phosphatidylinositol 4-kinase beta 1 (PI4KBETA1), partial

Code CSB-YP872291DOA
MSDS
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Source Yeast
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Code CSB-EP872291DOA
MSDS
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Source E.coli
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Code CSB-EP872291DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872291DOA
MSDS
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Source Baculovirus
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Code CSB-MP872291DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PI4KB1
Uniprot No.
Alternative Names
PI4KB1; PI4K; PI4KBETA1; At5g64070; MHJ24.5; Phosphatidylinositol 4-kinase beta 1; PI4-kinase beta 1; PtdIns-4-kinase beta 1; EC 2.7.1.67; Phosphatidylinositol 4-OH kinase beta1; AtPI4Kbeta1; PI-4Kbeta1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5-trisphosphate. Necessary for proper organization of the trans-Golgi network (TGN) and post-Golgi secretion in root hairs. Together with PI4KB2, required during polarized root hair expansion and pollen tube elongation. Functions redundantly with PI4KB2 upstream of the cold response phosphoinositide-dependent phospholipase C (PI-PLC) pathway.
Gene References into Functions
  1. PI4KIIIbeta1 and PI4KIIIbeta2 are genetically upstream of EDS1 and need functional salicylic acid biosynthesis and perception through NPR1 to express the dwarf phenotype. [PI4KIIIbeta1] PMID: 24758581
  2. PI-4Kbeta1 interacts with RabA4b through a novel homology domain, and interacts with a Ca2+ sensor through its NH2 terminus. PMID: 16567499
Subcellular Location
Cell membrane. Golgi apparatus, trans-Golgi network. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
PI3/PI4-kinase family, Type III PI4K subfamily
Tissue Specificity
Expressed constitutively in leaves, roots, flowers, and stems.
Database Links

KEGG: ath:AT5G64070

STRING: 3702.AT5G64070.1

UniGene: At.28255

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