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Recombinant Arabidopsis thaliana Phosphoglucan, water dikinase, chloroplastic (GWD3), partial

Recombinant Arabidopsis thaliana Phosphoglucan, water dikinase, chloroplastic (GWD3), partial

Code	CSB-YP744433DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP744433DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP744433DOA-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP744433DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP744433DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

GWD3

Uniprot No.

Q6ZY51

Alternative Names

GWD3; OK1; PWD; At5g26570; F9D12.1; F9D12.19; Phosphoglucan; water dikinase; chloroplastic; EC 2.7.9.5

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Mediates the incorporation of phosphate into starch-like phospho-alpha-glucan, mostly at the C-3 position of glucose units. Required for starch degradation, suggesting that the phosphate content of starch regulates its degradability.

Gene References into Functions

The expression and characterization of this phosphoglucan,water dikinase in A. thaliana. PMID: 15618411
An Arabidopsis thaliana gene encoding a homologue of the potato alpha-glucan, water dikinase GWD, previously known as R1, was identified by screening the Arabidopsis genome and named AtGWD3. [GWD3] PMID: 15686522
The role of alpha-glucan/water dikinase in the development of cold tolerance in conjunction with SEX1 protein is reported. PMID: 15894744

Subcellular Location

Plastid, chloroplast. Note=Starch granules during starch mobilization in darkness.

Protein Families

PEP-utilizing enzyme family

Tissue Specificity

In all starch containing tissues (e.g. roots, leaves, stems, inflorescence and siliques).

Database Links

KEGG: ath:AT5G26570

STRING: 3702.AT5G26570.1

UniGene: At.20942

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