Recombinant Arabidopsis thaliana Polyadenylate-binding protein RBP45B (RBP45B)

Code CSB-YP882807DOA
MSDS
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Source Yeast
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Code CSB-EP882807DOA
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Source E.coli
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Code CSB-EP882807DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP882807DOA
MSDS
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Source Baculovirus
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Code CSB-MP882807DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RBP45B
Uniprot No.
Alternative Names
RBP45B; At1g11650; F25C20.21; Polyadenylate-binding protein RBP45B; Poly(A)-binding protein RBP45B; RNA-binding protein 45B; AtRBP45B
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-405
Target Protein Sequence
MMQQPPPGGI LPHHAPPPSA QQQYGYQQPY GIAGAAPPPP QMWNPQAAAP PSVQPTTADE IRTLWIGDLQ YWMDENFLYG CFAHTGEMVS AKVIRNKQTG QVEGYGFIEF ASHAAAERVL QTFNNAPIPS FPDQLFRLNW ASLSSGDKRD DSPDYTIFVG DLAADVTDYI LLETFRASYP SVKGAKVVID RVTGRTKGYG FVRFSDESEQ IRAMTEMNGV PCSTRPMRIG PAASKKGVTG QRDSYQSSAA GVTTDNDPNN TTVFVGGLDA SVTDDHLKNV FSQYGEIVHV KIPAGKRCGF VQFSEKSCAE EALRMLNGVQ LGGTTVRLSW GRSPSNKQSG DPSQFYYGGY GQGQEQYGYT MPQDPNAYYG GYSGGGYSGG YQQTPQAGQQ PPQQPPQQQQ VGFSY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Heterogeneous nuclear ribonucleoprotein (hnRNP)-protein binding the poly(A) tail of mRNA and probably involved in some steps of pre-mRNA maturation.
Gene References into Functions
  1. RBP45b interacts with CBP20 and PAB8 proteins.RBP45b may play an important role in RNA metabolism, especially in aspects related to mRNA stability and translation initiation during stress conditions in plants. PMID: 28039930
Subcellular Location
Nucleus.
Protein Families
Polyadenylate-binding RBP45 family
Tissue Specificity
Expressed in roots, leaves, stems, flowers, siliques, and seedlings. Present in immature anther tissues (tapetum cells) and mature pollen grains.
Database Links

KEGG: ath:AT1G11650

STRING: 3702.AT1G11650.2

UniGene: At.19842

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