Recombinant Arabidopsis thaliana Polycomb group protein VERNALIZATION 2 (VRN2)

Code CSB-YP845096DOA
MSDS
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Source Yeast
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Code CSB-EP845096DOA
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Source E.coli
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Code CSB-EP845096DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP845096DOA
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Source Baculovirus
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Code CSB-MP845096DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VRN2
Uniprot No.
Alternative Names
VRN2; At4g16845; dl4450w; FCAALL.23Polycomb group protein VERNALIZATION 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-440
Target Protein Sequence
MCRQNCRAKS SPEEVISTDE NLLIYCKPVR LYNIFHLRSL GNPSFLPRCL NYKIGAKRKR KSRSTGMVVF NYKDCNNTLQ RTEVREDCSC PFCSMLCGSF KGLQFHLNSS HDLFEFEFKL LEEYQTVNVS VKLNSFIFEE EGSDDDKFEP FSLCSKPRKR RQRGGRNNTR RLKVCFLPLD SPSLANGTEN GIALLNDGNR GLGYPEATEL AGQFEMTSNI PPAIAHSSLD AGAKVILTTE AVVPATKTRK LSAERSEARS HLLLQKRQFY HSHRVQPMAL EQVMSDRDSE DEVDDDVADF EDRQMLDDFV DVNKDEKQFM HLWNSFVRKQ RVIADGHISW ACEVFSRFYE KELHCYSSLF WCWRLFLIKL WNHGLVDSAT INNCNTILEN CRNTSVTNNN NNSVDHPSDS NTNNNNIVDH PNDIKNKNNV DNKDNNSRDK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Polycomb group (PcG) protein. Plays a central role in vernalization by maintaining repressed the homeotic gene FLC, a floral repressor, after a cold treatment. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Associates constitutively along the whole FLC locus.
Subcellular Location
Nucleus.
Protein Families
VEFS (VRN2-EMF2-FIS2-SU(Z)12) family
Tissue Specificity
Weakly expressed. Expressed both during, and in the absence of vernalization.
Database Links

KEGG: ath:AT4G16845

STRING: 3702.AT4G16845.1

UniGene: At.11901

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