Recombinant Arabidopsis thaliana Polygalacturonase ADPG1 (ADPG1)

Code CSB-YP519529DOA
MSDS
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Source Yeast
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Code CSB-EP519529DOA
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Source E.coli
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Code CSB-EP519529DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519529DOA
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Source Baculovirus
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Code CSB-MP519529DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ADPG1
Uniprot No.
Alternative Names
ADPG1; PGDZAT; SAC70; At3g57510; T8H10.110; Polygalacturonase ADPG1; AtADPG1; PG ADPG1; EC 3.2.1.15; Pectinase ADPG1; Protein ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
24-431
Target Protein Sequence
LSSNVDD GYGHEDGSFE SDSLLKLNND DVLSLISSDE TTLEASTVSV SNFGAKGDGK TDDTQAFKKA WKKACSTNGV TTFLVPKGKT YLLKSTRFRG PCKSLRNFQI LGTLSASTKR SDYKDKNHWL ILEDVNNLSI DGGSTGIING NGKTWWQNSC KIDKSKPCTK APTALTLYNL KNLNVKNLRV KNAQQIQISI EKCNKVEVSN VEITAPGDSP NTDGIHITNT QNIRVSNSDI GTGDDCISIE DGTQNLQIFD LTCGPGHGIS IGSLGDDNSK AYVSGINVDG AKFSESDNGV RIKTYQGGSG TAKNIKFQNI RMENVKNPII IDQDYCDKDK CEDQESAVQV KNVVYKNISG TSATDVAITL NCSEKYPCQG IVLENVKIKG GTASCKNANV KNQGTVSPKC S
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Polygalacturonase involved in cell separation in the final stages of pod shatter and in anther dehiscence. Not involved in floral organ abscission.
Gene References into Functions
  1. ADPG1 and ADPG2 are essential for silique dehiscence. PMID: 19168715
Subcellular Location
Secreted, cell wall. Cytoplasm. Note=Released to the cell wall during maturation of the dehiscence zone.
Protein Families
Glycosyl hydrolase 28 family
Tissue Specificity
Expressed in flower buds and siliques, in the dehiscence zone of anthers (stomium cells) and maturing siliques. Expressed in stigma during pollen tube growth. Not expressed in seeds or in the floral part or leaf abscission zone but found at the junction b
Database Links

KEGG: ath:AT3G57510

STRING: 3702.AT3G57510.1

UniGene: At.26895

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