Recombinant Arabidopsis thaliana Probable WRKY transcription factor 28 (WRKY28)

Code CSB-YP823796DOA
MSDS
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Source Yeast
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Code CSB-EP823796DOA
MSDS
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Source E.coli
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Code CSB-EP823796DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP823796DOA
MSDS
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Source Baculovirus
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Code CSB-MP823796DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WRKY28
Uniprot No.
Alternative Names
WRKY28; At4g18170; F15J5.1; T9A21.10WRKY transcription factor 28; WRKY DNA-binding protein 28
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-318
Target Protein Sequence
MSNETRDLYN YQYPSSFSLH EMMNLPTSNP SSYGNLPSQN GFNPSTYSFT DCLQSSPAAY ESLLQKTFGL SPSSSEVFNS SIDQEPNRDV TNDVINGGAC NETETRVSPS NSSSSEADHP GEDSGKSRRK RELVGEEDQI SKKVGKTKKT EVKKQREPRV SFMTKSEVDH LEDGYRWRKY GQKAVKNSPY PRSYYRCTTQ KCNVKKRVER SFQDPTVVIT TYEGQHNHPI PTNLRGSSAA AAMFSADLMT PRSFAHDMFR TAAYTNGGSV AAALDYGYGQ SGYGSVNSNP SSHQVYHQGG EYELLREIFP SIFFKQEP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element.
Gene References into Functions
  1. These findings show how somatic cells in ovule primordia cooperatively use chromatin remodeling to restrict germ-line cell specification to a single cell. PMID: 29288215
  2. Transgenic lines overexpressing AtWRKY28 and AtWRKY75 showed enhanced resistance to oxalic acid and S. sclerotiorum, demonstrating that both AtWRKY28 and AtWRKY75 are novel positive regulators in Arabidopsis defense responses. PMID: 23749099
  3. WRKY28 specifically binds to the ICS1 promoter in vivo, most probably to one or both binding sites at position -460 and -445 upstream of the transcription start site. PMID: 21595875
Subcellular Location
Nucleus.
Protein Families
WRKY group II-c family
Database Links

KEGG: ath:AT4G18170

STRING: 3702.AT4G18170.1

UniGene: At.27349

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