Recombinant Arabidopsis thaliana Probable WRKY transcription factor 8 (WRKY8)

Code CSB-YP884280DOA
MSDS
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Source Yeast
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Code CSB-EP884280DOA
MSDS
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Source E.coli
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Code CSB-EP884280DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884280DOA
MSDS
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Source Baculovirus
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Code CSB-MP884280DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WRKY8
Uniprot No.
Alternative Names
WRKY8; At5g46350; MPL12.15; WRKY transcription factor 8; WRKY DNA-binding protein 8
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-326
Target Protein Sequence
MSHEIKDLNN YHYTSSYNHY NINNQNMINL PYVSGPSAYN ANMISSSQVG FDLPSKNLSP QGAFELGFEL SPSSSDFFNP SLDQENGLYN AYNYNSSQKS HEVVGDGCAT IKSEVRVSAS PSSSEADHHP GEDSGKIRKK REVRDGGEDD QRSQKVVKTK KKEEKKKEPR VSFMTKTEVD HLEDGYRWRK YGQKAVKNSP YPRSYYRCTT QKCNVKKRVE RSYQDPTVVI TTYESQHNHP IPTNRRTAMF SGTTASDYNP SSSPIFSDLI INTPRSFSND DLFRVPYASV NVNPSYHQQQ HGFHQQESEF ELLKEMFPSV FFKQEP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring stress-responsive cis-acting element. Functions as positive regulator of salt stress response. Binds the W box of LTI78/RD29A stress-response gene and directly regulates its transcription under salt stress. Functions antagonistically with VQ9 to regulate sodium and potassium homeostasis under salt stress by regulating the expression of downstream SOS (SALT OVERLY SENSITIVE) stress-responsive genes. The DNA-binding activity of WRKY8 is decreased by VQ9. Functions as negative regulator of basal resistance to the bacterial pathogen P. syringae and as positive regulator of resistance to the fungal pathogen to B. cinerea. Functions as positive regulator of defense response againt tobamovirus (TMV) by regulating both the abscisic acid and ethylene signaling pathways. Positively regulates ABI4 expression and negatively modulates ACS6 and ERF104 expression by directly binding to the W box consensus motifs within their promoters.
Gene References into Functions
  1. Data indicate that WRKY8 interacts with VQ9 (At1g78310) in the nucleus of plant cells, and binds the promoter of RD29A (AT5G52310), in vivo. PMID: 23451802
  2. WRKY8 is involved in the defense response against TMV-cg through the direct regulation of the expression of ABI4, ACS6, and ERF104 and may mediate the crosstalk between ABA and ethylene signaling during the TMV-cg-Arabidopsis interaction. PMID: 23650359
  3. WRKY8 is a negative regulator of basal resistance to P. syringae and positive regulator to B. cinerea. PMID: 20367464
Subcellular Location
Nucleus.
Protein Families
WRKY group II-c family
Tissue Specificity
Highly expressed in roots and at lower levels in rosette leaves, cauline leaves, stems, flowers and siliques.
Database Links

KEGG: ath:AT5G46350

STRING: 3702.AT5G46350.1

UniGene: At.26517

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