Recombinant Arabidopsis thaliana Probable protein phosphatase 2C 25 (At2g30020)

Code CSB-YP530513DOA
MSDS
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Source Yeast
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Code CSB-EP530513DOA
MSDS
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Source E.coli
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Code CSB-EP530513DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP530513DOA
MSDS
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Source Baculovirus
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Code CSB-MP530513DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
At2g30020
Uniprot No.
Alternative Names
At2g30020; F23F1.6; Probable protein phosphatase 2C 25; AtPP2C25; EC 3.1.3.16; Protein phosphatase AP2C1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-396
Target Protein Sequence
MSCSVAVCNS PVFSPSSSLF CNKSSILSSP QESLSLTLSH RKPQTSSPSS PSTTVSSPKS PFRLRFQKPP SGFAPGPLSF GSESVSASSP PGGVLKRKRP TRLDIPIGVA GFVAPISSSA AVAATPREEC REVEREGDGY SVYCKRGRRE AMEDRFSAIT NLHGDRKQAI FGVYDGHGGV KAAEFAAKNL DKNIVEEVVG KRDESEIAEA VKHGYLATDA SFLKEEDVKG GSCCVTALVN EGNLVVSNAG DCRAVMSVGG VAKALSSDHR PSRDDERKRI ETTGGYVDTF HGVWRIQGSL AVSRGIGDAQ LKKWVIAEPE TKISRIEHDH EFLILASDGL WDKVSNQEAV DIARPLCLGT EKPLLLAACK KLVDLSASRG SSDDISVMLI PLRQFI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein phosphatase that negatively regulates defense respones. Inactivates MPK4 and MPK6 MAP kinases involved in stress and defense signaling.
Gene References into Functions
  1. This study pinpoints the specificity of MAPK regulation by the different MAPK phosphatases AP2C1 and MKP1, which control the same MAPK substrates, nevertheless leading to different downstream events. PMID: 28062592
  2. Phosphatase AP2C1, as well as AP2C1-targeted MPK3 and MPK6, are important regulators of plant-nematode interaction, where the co-ordinated action of these signalling components ensures the timely activation of plant defence. PMID: 26438412
  3. These results demonstrate a key role for the AP2C1 phosphatase in regulating stress hormone levels, defense responses, and MAPK activities in Arabidopsis. [AP2C1] PMID: 17630279
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
PP2C family
Database Links

KEGG: ath:AT2G30020

STRING: 3702.AT2G30020.1

UniGene: At.19796

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