Recombinant Arabidopsis thaliana Probable transcriptional regulator RABBIT EARS (RBE)

Code CSB-YP873271DOA
MSDS
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Source Yeast
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Code CSB-EP873271DOA
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Source E.coli
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Code CSB-EP873271DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP873271DOA
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Source Baculovirus
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Code CSB-MP873271DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RBE
Uniprot No.
Alternative Names
RBE; At5g06070; K16F4.3; Probable transcriptional regulator RABBIT EARS
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-226
Target Protein Sequence
MMDRGECLMS MKLRPMVTRP SSDGTLFWPF REERAFASAE EYGGGGGCMW PPRSYSCSFC GREFKSAQAL GGHMNVHRRD RARLKQQSLS PSSTDQATPP ECDRQQQVLD VGSKVLVQEE TRKPNGTKRE ISDVCNNNVL ESSMKRYEHD NGEVKTDLSV GLLSTEFDPR KKQLINGSSS SWKRAKTDVS RFPMMLGLVI GISEINGHHE ELDLELRLGA DPPKVN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable transcriptional regulator essential for petal development. Required for the early development of the organ primordia of the second whorl. Acts downstream of AP1 and PTL.
Gene References into Functions
  1. regulates the transcription of TCP4 during petal development PMID: 27838638
  2. RBE promotes cell division and negatively regulates the expression of TCP5, TCP13, and TCP17 in young petals. PMID: 26073137
  3. restriction of expression to the petal precursor cells crucial for flower development PMID: 24366683
  4. These results indicate that one role of RBE is to fine-tune miR164 expression to regulate the CUC1 and CUC2 effector genes, which, in turn, regulate developmental events required for sepal and petal organogenesis. PMID: 22573623
  5. RBE acts to maintain two different types of spatial boundaries in young flowers: boundaries between organ primordia within a whorl and boundaries of homeotic gene expression between whorls. PMID: 16412084

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Subcellular Location
Nucleus.
Tissue Specificity
Strongly expressed in inflorescences and flowers, and weakly in siliques, seedlings and roots. In flowers, it is expressed in petal primordia and their precursor cells. Also expressed in the lateral root caps and the basal cells of lateral roots.
Database Links

KEGG: ath:AT5G06070

STRING: 3702.AT5G06070.1

UniGene: At.32906

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