Recombinant Arabidopsis thaliana Probable xyloglucan endotransglucosylase/hydrolase protein 31 (XTH31)

Code CSB-YP309378DOA
MSDS
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Source Yeast
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Code CSB-EP309378DOA
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Source E.coli
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Code CSB-EP309378DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP309378DOA
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Source Baculovirus
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Code CSB-MP309378DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
XTH31
Uniprot No.
Alternative Names
XTH31; ATXG; XTR8; At3g44990; F14D17.60Xyloglucan endotransglucosylase/hydrolase protein 31; At-XTH31; AtXTR8; XTH-31; EC 2.4.1.207; EC 3.2.1.151
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
21-293
Target Protein Sequence
QRSPSPGYYP SSRVPTSPFD REFRTLWGSQ HQRREQDVVT LWLDKSTGSG FKSLRPYRSG YFGASIKLQP GFTAGVDTSL YLSNNQEHPG DHDEVDIEFL GTTPGKPYSL QTNVFVRGSG DRNVIGREMK FTLWFDPTQD FHHYAILWNP NQIVFFVDDV PIRTYNRKNE AIFPTRPMWV YGSIWDASDW ATENGRIKAD YRYQPFVAKY KNFKLAGCTA DSSSSCRPPS PAPMRNRGLS RQQMAALTWA QRNFLVYNYC HDPKRDHTQT PEC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues. Involved in the accumulation of hemicelluloses. Has a high XEH activity and only a slight XET activity in vitro, but the main in planta activity seems to be XET, thus controlling aluminum sensitivity. Acceptor preferences are XXXGol = XXFGol > XXLGol > XLLGol = XLFGol.
Gene References into Functions
  1. catalytic capacities of two aluminium-repressed Arabidopsis thaliana xyloglucan endotransglucosylase/hydrolases, XTH15 and XTH31 PMID: 25446234
  2. XTH31 affects Al sensitivity by modulating cell wall xyloglucan content and Al binding capacity. PMID: 23204407
Subcellular Location
Secreted, cell wall. Secreted, extracellular space, apoplast. Cell membrane.
Protein Families
Glycosyl hydrolase 16 family, XTH group 3 subfamily
Tissue Specificity
Predominantly expressed in root. Weakly expressed in influorescence stems. Expressed in root tips and elongation zones, stems, young leaves, flowers and siliques. Expressed in root, hypocotyl, and etiolated whole seedlings.
Database Links

KEGG: ath:AT3G44990

STRING: 3702.AT3G44990.1

UniGene: At.20372

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