Recombinant Arabidopsis thaliana Profilin-5 (PRO5)

Code CSB-YP880709DOA
MSDS
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Source Yeast
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Code CSB-EP880709DOA
MSDS
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Source E.coli
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Code CSB-EP880709DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880709DOA
MSDS
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Source Baculovirus
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Code CSB-MP880709DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PRO5
Uniprot No.
Alternative Names
PRF3; PRO5; At5g56600; MIK19.4; Profilin-3; AtPRF3; AthPRF3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
2-131
Target Protein Sequence
SWQTYVDDH LMCDVAGNRL TAAAILGQDG SVWAQSNNFP QVKPEEIQGI KDDFTTPGTL APTGLFLGGN KYMVIQGEPN AVIRGKKGAG GVTIKKTTLA LVFGIYDEPM TPGQCNMVVE NLGEYLIESG L
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to actin monomers and regulates the organization of the actin cytoskeleton. Can increase the critical concentration (Cc) of actin assembly in vitro. Acts as downstream effector of the hydrogen sulfide signaling to regulate the assembly and depolymerization of F-actin. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations (Probable). Binding to the poly-proline motif of formin induces oligomerization of PRF3. PRF3 oligomers inhibit formin-mediated actin assembly to modulate plant immunity triggered by pathogen-associated molecular patterns (PAMPs).
Gene References into Functions
  1. These results suggest that the dwarf phenotype of the PRF3 overexpression seedlings may be related to a reduction in cell length and F-actin rearrangement. PMID: 23052593
Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Profilin family
Tissue Specificity
Expressed in roots, rosette leaves, cauline leaves, stems and flowers.
Database Links

KEGG: ath:AT5G56600

STRING: 3702.AT5G56600.1

UniGene: At.9264

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