Recombinant Arabidopsis thaliana Protease Do-like 1, chloroplastic (DEGP1)

Code CSB-YP515782DOA
MSDS
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Source Yeast
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Code CSB-EP515782DOA
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Source E.coli
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Code CSB-EP515782DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP515782DOA
MSDS
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Source Baculovirus
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Code CSB-MP515782DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DEGP1
Uniprot No.
Alternative Names
DEGP1; DEG1; DEGP; At3g27925; K16N12.18Protease Do-like 1; chloroplastic; EC 3.4.21.-; Protein DEGRADATION OF PERIPLASMIC PROTEINS 1; DEGP PROTEASE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
106-439
Target Protein Sequence
FVVST PKKLQTDELA TVRLFQENTP SVVYITNLAV RQDAFTLDVL EVPQGSGSGF VWDKQGHIVT NYHVIRGASD LRVTLADQTT FDAKVVGFDQ DKDVAVLRID APKNKLRPIP VGVSADLLVG QKVFAIGNPF GLDHTLTTGV ISGLRREISS AATGRPIQDV IQTDAAINPG NSGGPLLDSS GTLIGINTAI YSPSGASSGV GFSIPVDTVG GIVDQLVRFG KVTRPILGIK FAPDQSVEQL GVSGVLVLDA PPSGPAGKAG LQSTKRDGYG RLVLGDIITS VNGTKVSNGS DLYRILDQCK VGDEVTVEVL RGDHKEKISV TLEPKPDES
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine protease that is required at high temperature. May be involved in the degradation of damaged proteins. In vivo, can degrade beta-casein.
Gene References into Functions
  1. Photoinhibitory treatment of Arabidopsis thaliana leaves activates Deg1-mediated proteolysis of the PSII core and antenna subunits. PMID: 21877139
  2. Structural and biochemical analyses reveal that the inactive Deg1 monomer is transformed into the proteolytically active hexamer at acidic pH. PMID: 21532594
  3. Deg1 directly interacts with PSII reaction center D2 protein, and functions in the assembly of PSII. PMID: 20088900
  4. These results suggest that Deg1 cooperates with the stroma-exposed proteases FtsH and Deg2 in degrading D1 protein during repair from photoinhibition by cleaving lumen-exposed regions of the protein.[Deg1] PMID: 17351117
Subcellular Location
Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side.
Protein Families
Peptidase S1C family
Database Links

KEGG: ath:AT3G27925

STRING: 3702.AT3G27925.1

UniGene: At.47511

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