Recombinant Arabidopsis thaliana Protein BREVIS RADIX (BRX)

Code CSB-YP615069DOA
MSDS
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Source Yeast
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Code CSB-EP615069DOA
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Source E.coli
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Code CSB-EP615069DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP615069DOA
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Source Baculovirus
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Code CSB-MP615069DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BRX
Uniprot No.
Alternative Names
BRX; At1g31880; F5M6.11Protein BREVIS RADIX; AtBRX
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-344
Target Protein Sequence
MFSCIACTKA DGGEEVEHGA RGGTTPNTKE AVKSLTIQIK DMALKFSGAY KQCKPCTGSS SSPLKKGHRS FPDYDNASEG VPYPFMGGSA GSTPAWDFTN SSHHPAGRLE SKFTSIYGND RESISAQSCD VVLDDDGPKE WMAQVEPGVH ITFASLPTGG NDLKRIRFSR EMFDKWQAQR WWGENYDKIV ELYNVQRFNR QALQTPARSD DQSQRDSTYS KMDSARESKD WTPRHNFRPP GSVPHHFYGG SSNYGPGSYH GGPPMDAART TTSSRDDPPS MSNASEMQAE WIEEDEPGVY ITIRQLSDGT RELRRVRFSR ERFGEVHAKT WWEQNRERIQ TQYL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a regulator of cell proliferation and elongation in the root and shoot. Regulates roots architecture and primary root protophloem differentiation. BRX, BAM3, and CLE45 act together to regulate the transition of protophloem cells from proliferation to differentiation, thus impinging on postembryonic growth capacity of the root meristem. Probable transcription regulator. Regulated by the auxin response factor ARF5. Polarly localized in vascular cells and subject to endocytic recycling. Required for CPD expression and for correct nuclear auxin response. Mediates cross-talk between the auxin and brassinosteroid pathways. BRX is a target for auxin-induced, proteasome-mediated degradation.
Gene References into Functions
  1. a novel brx allele from another acidic sampling site demonstrates the existence of independently maintained brx loss-of-function alleles in nature and supports the notion that they are advantageous in acidic soil pH conditions. PMID: 23041192
  2. These results demonstrate a ubiquitous, although quantitatively variable role of BRX in modulating the growth rate in both the root and shoot. PMID: 20649916
  3. AtBRX(Lc-0) allele seems to contribute to natural variation in root growth vigor and provides a rare example of an experimentally confirmed, hyperactive allelic variant. PMID: 20404146
  4. The brx phenotype results from a root-specific deficiency of brassinosteroid and is due to reduced, BRX-dependent expression of a rate-limiting enzyme in brassinosteroid biosynthesis [BRX] PMID: 17006513
  5. A brx loss-of-function allele, which was found in a natural accession, results in a highly branched but poorly elongated root system. PMID: 18627499
  6. BRX is associated with cytokinin-mediated inhibition of lateral root initiation. PMID: 19037657
  7. A loss-of-function allele of BREVIS RADIX (BRX) in the Columbia background, named brx-2, which shows enhanced response to ABA-mediated inhibition of root growth, was identified. PMID: 19201913
  8. BRX might provide a contextual readout to synchronize cellular growth with the auxin concentration gradient across the root tip. PMID: 19465596

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Subcellular Location
Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
BRX family
Tissue Specificity
Expressed in the developing protophloem up to the elongation zone in root meristem of young seedlings, in the columella and the phloem vasculature throughout the root and in the phloem vasculature in the shoot. Detected in the shoot meristem and in primor
Database Links

KEGG: ath:AT1G31880

STRING: 3702.AT1G31880.1

UniGene: At.40252

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