Recombinant Arabidopsis thaliana Protein EARLY FLOWERING 4 (ELF4)

Code CSB-YP515340DOA
MSDS
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Source Yeast
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Code CSB-EP515340DOA
MSDS
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Source E.coli
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Code CSB-EP515340DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP515340DOA
MSDS
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Source Baculovirus
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Code CSB-MP515340DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ELF4
Uniprot No.
Alternative Names
ELF4; ARR08; ARR16; ARR19; ARR44; ARR80; At2g40080; T28M21.24Protein EARLY FLOWERING 4; Protein ARRHYTHMIC 44
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-111
Target Protein Sequence
MKRNGETKRR RNVAEEAEQG EDPAMWENLD RNFRQVQSVL DRNRSLIQQV NDNHQSRMAD NMSKNVALIQ ELNGNISKVV NMYSDLNTSF SSGFHGGKNG HDGGGAAGTR A
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the central CCA1/LHY-TOC1 feedback loop in the circadian clock that promotes clock accuracy and is required for sustained rhythms in the absence of daily light/dark cycles. Part of a corepressor complex consisting of ELF4, ELF3, and LUX involved in the transcriptional regulation of APRR9. Increases ELF3 nuclear distribution and localization in nuclear bodies. Required for responsiveness to continuous red, by regulating phytochrome B (phyB) signaling (including during seedling de-etiolation) and gene expression. Mediates both entrainment to an environmental cycle and circadian rhythm sustainability under constant conditions. Controls flowering time. Necessary for light-induced expression of both CCA1 and LHY.
Gene References into Functions
  1. GI and ELF4 have differentially dominant influences on circadian physiological outputs at dusk and dawn, respectively. PMID: 22328721
  2. ELF4 is required to regulate the expression of CCA1 and TOC1 and can be considered a component of the central CCA1/LHY-TOC1 feedback loop in the plant circadian clock. PMID: 17384164
Subcellular Location
Nucleus.
Protein Families
EARLY FLOWERING 4 family
Database Links

KEGG: ath:AT2G40080

STRING: 3702.AT2G40080.1

UniGene: At.14672

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