Recombinant Arabidopsis thaliana Protein LHY (LHY), partial

Code CSB-YP740824DOA
MSDS
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Source Yeast
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Code CSB-EP740824DOA
MSDS
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Source E.coli
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Code CSB-EP740824DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP740824DOA
MSDS
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Source Baculovirus
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Code CSB-MP740824DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LHY
Uniprot No.
Alternative Names
LHY; At1g01060; T25K16.6Protein LHY; MYB-related transcription factor LHY; Protein LATE ELONGATED HYPOCOTYL
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in the circadian clock. Binds to the promoter region of APRR1/TOC1 and TCP21/CHE to repress their transcription. Represses both CCA1 and itself.
Gene References into Functions
  1. Transcriptional co-regulators PRR9, PRR7 and PRR5 inhibit morning loop LHY and CCA1 expression by binding to their promoters PMID: 24267177
  2. Rhythmic expression of LHY under light-dark cycling conditions correlates with histone modification. PMID: 22878891
  3. Interaction of Arabidopsis DET1 with LHY in mediating transcriptional repression in the plant circadian clock PMID: 21884973
  4. A conserved G-Box motif contributes to the rhythmic expression of LHY. PMID: 19789276
  5. Accumulation of the LHY protein in the lhy-1 mutant was altered by the det1-1 mutation and through the proteasome pathway. PMID: 15988568

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in leaves, roots, stems, flowers and siliques.
Database Links

KEGG: ath:AT1G01060

STRING: 3702.AT1G01060.1

UniGene: At.25466

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