Recombinant Arabidopsis thaliana Protein MID1-COMPLEMENTING ACTIVITY 1 (MCA1), partial

Code CSB-YP813155DOA1
MSDS
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Source Yeast
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Code CSB-EP813155DOA1
MSDS
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Source E.coli
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Code CSB-EP813155DOA1-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP813155DOA1
MSDS
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Source Baculovirus
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Code CSB-MP813155DOA1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MCA1
Uniprot No.
Alternative Names
MCA1; At4g35920; T19K4.50; Protein MID1-COMPLEMENTING ACTIVITY 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Calcium-permeable stretch-activated channel component. Involved in mechano-stimulated calcium uptake mechanism and in mechanosensing in the primary root.
Gene References into Functions
  1. MCA1 is involved in the cold-induced elevation of [Ca2+]cyt, cold tolerance, and CBF/DREB1-independent cold signaling. PMID: 29323146
  2. MCA1 encodes a possible mechanosensitive channel with a conductance of 34 pS. PMID: 22751361
  3. mca1-null mca2-null double mutants are defective in Ca2+ uptake from the roots and have a slow growth phenotype. PMID: 20097794
Subcellular Location
Cell membrane; Single-pass membrane protein.
Tissue Specificity
Expressed in roots, leaves, stems, flowers and siliques. Expressed in vascular tissues of cotyledons, leaves and primary root, in the promeristem and adjacent elongation zone of the primary root and in the shoot apical meristem. Detected in the stele and
Database Links

KEGG: ath:AT4G35920

STRING: 3702.AT4G35920.1

UniGene: At.19123

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