Recombinant Arabidopsis thaliana Protein MKS1 (MKS1)

Code CSB-YP839698DOA
MSDS
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Source Yeast
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Code CSB-EP839698DOA
MSDS
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Source E.coli
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Code CSB-EP839698DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839698DOA
MSDS
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Source Baculovirus
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Code CSB-MP839698DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MKS1
Uniprot No.
Alternative Names
MKS1; VQ21; At3g18690; MVE11.5Protein MKS1; Protein MAP kinase 4 substrate 1; VQ motif-containing protein 21; AtVQ21
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-222
Target Protein Sequence
MDPSEYFAGG NPSDQQNQKR QLQICGPRPS PLSVHKDSHK IKKPPKHPAP PPNRDQPPPY IPREPVVIYA VSPKVVHATA SEFMNVVQRL TGISSGVFLE SGGGGDVSPA ARLASTENAS PRGGKEPAAR DETVEINTAM EEAAEFGGYA PGILSPSPAL LPTASTGIFS PMYHQGGMFS PAIPLGLFSP AGFMSPFRSP GFTSLVASPT FADFFSHIWD QD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulator of plant defense response. May contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.
Gene References into Functions
  1. This study evaluates the effect of manipulating MAP kinase 4 nuclear substrate 1 (MKS1) expression in Kalanchoe blossfeldiana and Petunia hybrida. PMID: 25082411
  2. MKS1 interacts with the transcription factor WRKY33, which in turn controls the regulation of PAD3 and CYP71A13, two genes, required for proper resistance to B. cinerea. PMID: 21900742
  3. MKS1 is required for full salicylic acid-dependent resistance in MAP kinase 4 mutants PMID: 15990873
  4. three of the twelve Ser-Pro serine residues (Ser72, Ser108, Ser120) are in the phosphorylated form. PMID: 17702678
Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT3G18690

STRING: 3702.AT3G18690.1

UniGene: At.6380

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