Recombinant Arabidopsis thaliana Protein NDR1 (NDR1)

Code CSB-YP527398DOA
MSDS
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Source Yeast
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Code CSB-EP527398DOA
MSDS
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Source E.coli
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Code CSB-EP527398DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP527398DOA
MSDS
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Source Baculovirus
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Code CSB-MP527398DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NDR1
Uniprot No.
Alternative Names
NDR1; At3g20600; K10D20.24Protein NDR1; Non-race specific disease resistance protein 1; AtNDR1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
39-195
Target Protein Sequence
DK PKCSIQNFFI PALGKDPNSR DNTTLNFMVR CDNPNKDKGI YYDDVHLNFS TINTTKINSS ALVLVGNYTV PKFYQGHKKK AKKWGQVKPL NNQTVLRAVL PNGSAVFRLD LKTQVRFKIV FWKTKRYGVE VGADVEVNGD GVKAQKKGIK MKKSD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in disease resistance. Required for resistance conferred by multiple R genes recognizing different bacterial and oomycete pathogen isolates like avirulent P.syringae or H.parasitica (downy mildew). Required for the establishment of hypersensitive response (HR) and systemic acquired resistance (SAR) after infection with the bacterial pathogen P.syringae DC3000 carrying avrRpt2. Required for resistance to the soilborne fungus V.longisporum. Interaction with RIN4 is required for the activation of the R gene RPS2 and RPS2-mediated resistance.
Gene References into Functions
  1. NDR1 plays a broad role both in mediating primary cellular functions in Arabidopsis through maintaining the integrity of the cell wall-plasma membrane connection and as a key signaling component of these responses during pathogen infection. PMID: 21398259
  2. The induction of defence responses and disease resistance to X. campestris pv. campestris strain 8004 requires NDR1 , RAR1 and SGT1b, suggesting that effector-triggered immunity plays a large role in resistance to this strain. PMID: 21029323
  3. Sequence analysis and mass spectrometry suggest that NDR1 is localized to the PM via a C-terminal glycosylphosphatidyl-inositol (GPI) anchor. PMID: 15447649
  4. demonstrate that the RIN4-NDR1 interaction occurs on the cytoplasmically localized N-terminal portion of NDR1 and that this interaction is required for the activation of resistance signaling following infection by P. syringae PMID: 17012600
  5. Mutations in NDR1 abolished the enhanced resistance of dnd mutants against Pseudomonas syringae pv. tomato and Hyaloperonospora parasitica but not Botrytis cinerea. PMID: 18785824

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Database Links

KEGG: ath:AT3G20600

STRING: 3702.AT3G20600.1

UniGene: At.38209

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