Recombinant Arabidopsis thaliana Protein SHORT-ROOT (SHR), partial

Code CSB-YP866031DOA
MSDS
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Source Yeast
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Code CSB-EP866031DOA
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Source E.coli
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Code CSB-EP866031DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866031DOA
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Source Baculovirus
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Code CSB-MP866031DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SHR
Uniprot No.
Alternative Names
SHR; SGR7; At4g37650; F19F18.140; Protein SHORT-ROOT; AtSHR; GRAS family protein 26; AtGRAS-26; Protein SHOOT GRAVITROPISM 7
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor required for quiescent center cells specification and maintenance of surrounding stem cells, and for the asymmetric cell division involved in radial pattern formation in roots. Essential for both cell division and cell specification. Regulates the radial organization of the shoot axial organs and is required for normal shoot gravitropism. Directly controls the transcription of SCR, and when associated with SCR, of MGP, RLK, TRI, NUC and SCL3.
Gene References into Functions
  1. This study showed that INDETERMINATE DOMAIN PROTEIN (ID) binding sequences have a crucial role in the regulation of SCARECROW and SHORT-ROOT expression.[SHR] PMID: 28324206
  2. interaction of KinG with SHR allows for the formation of stable movement complexes that facilitate the cell-to-cell transport of SHR. PMID: 29122988
  3. Arabidopsis thaliana SHORT ROOT protein SHR gene, rice OsSHR1 and OsSHR2, could complement the A. thaliana shr mutant. PMID: 28263767
  4. all of the SHR proteins function as mobile signals PMID: 25352666
  5. The results together suggest that SHR regulates vascular patterning, but not root apical meristematic activity, through cytokinin homeostasis. PMID: 22476466
  6. The SHORT-ROOT protein acts as a mobile, dose-dependent signal in patterning the ground tissue. PMID: 22826238
  7. SHR functions in plant growth and development as a regulator of cell division and meristem activity not only in the roots but also in the shoots. PMID: 22194939
  8. One mechanism by which SHR controls vascular patterning is the regulation of cytokinin homeostasis. PMID: 21951467
  9. SHR regulates a wide array of Arabidopsis root-related developmental processes. PMID: 21030506
  10. SHR plays a central role in the root vascular system to control patterning processes, possibly regulated by longitudinal and radial signals. PMID: 20680487
  11. SHR regulates the spatiotemporal activation of specific genes involved in cell division PMID: 20596025
  12. The role of SHORT-ROOT and SCARECROW proteins in the maturation of A. thaliana roots, including cell organization, is reported. PMID: 15955927
  13. Combined analysis of global expression profiles of A. thaliana after modulating SHR activity in order to identify the direct targets of SHR is reported. PMID: 16640459
  14. SHR movement from the stele is required for proper patterning of the root. Multiple domains are required for SHR movement and for proper sub-cellular localization. PMID: 19000160

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Subcellular Location
Cytoplasm. Nucleus. Early endosome. Late endosome. Recycling endosome.
Protein Families
GRAS family
Tissue Specificity
Expressed in the stele and the quiescent center. Not detected in the ground tissue cell lineage. The SHR protein moves from the stele to a single layer of adjacent cells, where it enters the nucleus.
Database Links

KEGG: ath:AT4G37650

STRING: 3702.AT4G37650.1

UniGene: At.2816

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