Recombinant Arabidopsis thaliana Protein TOC75-3, chloroplastic (TOC75-3), partial

Code CSB-YP871154DOA
MSDS
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Source Yeast
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Code CSB-EP871154DOA
MSDS
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Source E.coli
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Code CSB-EP871154DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871154DOA
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Source Baculovirus
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Code CSB-MP871154DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TOC75-3
Uniprot No.
Alternative Names
TOC75-3; TOC75; At3g46740; T6H20.230; Protein TOC75-3; chloroplastic; 75 kDa translocon at the outer-envelope-membrane of chloroplasts 3; AtTOC75-III
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential protein. Mediates the insertion of proteins targeted to the outer membrane of chloroplasts. Required for the import of protein precursors into chloroplasts. Forms the voltage-dependent preprotein translocation channels (hydrophilic beta barrel) of the TOC complex in the chloroplastic outer membrane.
Gene References into Functions
  1. Toc75 polypeptide transport-associated (POTRA) domains are located in the intermembrane space of the chloroplast envelope by immunogold electron microscopy. PMID: 27388682
  2. Data indicate that OEP80-T7(atToc75-V) and atToc75-III showed distinct migration patterns on blue native-PAGE. PMID: 22094888
  3. the POTRA domains of both Toc75-III and Toc75-V are exposed to the cytoplasm PMID: 21825140
  4. SPase I converted Toc75 precursor to its mature form in vitro. PMID: 16275749
  5. Embryo development in the atToc75-III mutant, lacking the pore forming component of the translocase, was arrested extremely early at the two-cell stage. PMID: 16435266
  6. atTOC75-III of the Toc75 (translocon at the outer envelope membrane of chloroplasts, 75 kD) gene family is essential for plant viability, and is expressed at its highest level in young, rapidly expanding tissues PMID: 15908591

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Subcellular Location
Plastid, chloroplast outer membrane; Multi-pass membrane protein.
Protein Families
TOC75 family
Tissue Specificity
Mostly expressed in young and actively dividing photosynthetic tissues and, to a lower extent, in old leaves and roots. Particularly low levels in leaves after etiolation.
Database Links

KEGG: ath:AT3G46740

STRING: 3702.AT3G46740.1

UniGene: At.3633

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