Recombinant Arabidopsis thaliana Protein UNUSUAL FLORAL ORGANS (UFO)

Code CSB-YP657186DOA
MSDS
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Source Yeast
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Code CSB-EP657186DOA
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Source E.coli
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Code CSB-EP657186DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657186DOA
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Source Baculovirus
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Code CSB-MP657186DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UFO
Uniprot No.
Alternative Names
UFO; FBX1; At1g30950; F17F8.16; Protein UNUSUAL FLORAL ORGANS; F-box only protein 1; AtFBX1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-442
Target Protein Sequence
MDSTVFINNP SLTLPFSYTF TSSSNSSTTT STTTDSSSGQ WMDGRIWSKL PPPLLDRVIA FLPPPAFFRT RCVCKRFYSL LFSNTFLETY LQLLPLRHNC FLFFKHKTLK SYIYKRGGTN DDDSNKAEGF LFDPNEIRWY RLSFAYIPSG FYPSGSSGGL VSWVSEEAGL KTILLCNPLV GSVSQLPPIS RPRLFPSIGL SVTPTSIDVT VAGDDLISPY AVKNLSSESF HVDAGGFFSL WAMTSSLPRL CSLESGKMVY VQGKFYCMNY SPFSVLSYEV TGNRWIKIQA PMRRFLRSPS LLESKGRLIL VAAVEKSKLN VPKSLRLWSL QQDNATWVEI ERMPQPLYTQ FAAEEGGKGF ECVGNQEFVM IVLRGTSLQL LFDIVRKSWL WVPPCPYSGS GGGSSGGGSD GEVLQGFAYD PVLTTPVVSL LDQLTLPFPG VC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Considered as a meristem identity factor required for normal growth of the young floral meristem. Acts together with LEAFY to positively regulate the B class floral homeotic genes APETALA3 and PISTILLATA. In this way, operates as a region-specific regulator for petal and stamen development. Alternatively, may play a role as a negative regulator of the C class floral homeotic genes. Interacts together with the SKP1-like protein ASK1 to form a ubiquitin E3 ligase complex and could indirectly promote the ubiquitination and degradation of specific proteins controlling the floral primordia development like repressors of B class floral homeotic genes.
Gene References into Functions
  1. UFO alters leaf development and produces ectopic floral and inflorescence meristems PMID: 24376756
  2. This study suggests that UFO and LFY are jointly required in the inflorescence meristem to both promote floral meristem development and inhibit, in a non-cell autonomous manner, growth of the bract. PMID: 16244866
  3. UFO physically interacts with LFY, a necessary interaction to recruit UFO to the AP3 promoter. PMID: 18287201
Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT1G30950

STRING: 3702.AT1G30950.1

UniGene: At.51845

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