Recombinant Arabidopsis thaliana Protein XAP5 CIRCADIAN TIMEKEEPER (XCT)

Code CSB-YP818134DOA
MSDS
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Source Yeast
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Code CSB-EP818134DOA
MSDS
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Source E.coli
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Code CSB-EP818134DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818134DOA
MSDS
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Source Baculovirus
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Code CSB-MP818134DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
XCT
Uniprot No.
Alternative Names
XCT; At2g21150; F26H11.9; Protein XAP5 CIRCADIAN TIMEKEEPER
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-337
Target Protein Sequence
MSGMGDGYVG TAQDAVRIRR LQKQREAERK KIQELKSKSA SGNDQSGLLQ FGTSSCEILD TAFKKETVGL VTREEYVEKR VNIRNKFEEE EKEKLQKLQQ EEEELQLEKR NKKRKIKGSS RLSFAEDFEN GSDEDDGENK SSGTGNLRCG KLGKDPSVET NFLPDSEREA EEQAERERLK KQWLREQEQI KNEPLEITYS YWDGTGHRRV IQVRKGDPIG NFLRAVQQQL APDFREIRTA SVENLLYVKE DLIIPHQHSF YELIINKARG KSGPLFHFDV HEDVRTIADA TIEKDESHAG KVVERHWYEK NKHIFPASRW EIYDPTKKWE RYTVHGD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in light regulation of the circadian clock and photomorphogenesis. May play a global role in coordinating growth in response to the light environment. Acts as a light quality sensor directing both negative and positive transcriptional regulation. Inhibits growth in red light but promote growth in blue light. Inhibits clock gene expression in diurnal cycles. Plays no role in the control of flowering time.
Gene References into Functions
  1. XCT is a negative regulator of the blue light-mediated ethylene response in aerial tissues. PMID: 21163961
  2. Data have identified XAP5 CIRCADIAN TIMEKEEPER (XCT), an Arabidopsis thaliana gene important for light regulation of the circadian clock and photomorphogenesis. [XCT] PMID: 18515502
Subcellular Location
Nucleus.
Protein Families
FAM50 family
Tissue Specificity
Expressed in leaves stems, flowers, roots, trichomes and hypocotyls.
Database Links

KEGG: ath:AT2G21150

STRING: 3702.AT2G21150.1

UniGene: At.44017

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