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Recombinant Arabidopsis thaliana Protein argonaute 6 (AGO6), partial

Recombinant Arabidopsis thaliana Protein argonaute 6 (AGO6), partial

Code	CSB-YP528633DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP528633DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP528633DOA-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP528633DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP528633DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

AGO6

Uniprot No.

O48771

Alternative Names

AGO6; At2g32940; T21L14.12Protein argonaute 6

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Involved in transcriptional gene silencing (TGS). Component of the RISC complex that associate with the small interfering RNA (siRNA) pathway involved in direct cytosine methylation at endogenous DNA repeats. Required for the accumulation of specific siRNAs derived from transgene and heterochromatin-related endogenous loci. Involved in RNA-directed DNA methylation (RdDM) at specific endogenous loci. Probably not required for the accumulation of siRNAs derived from transgene inverted repeats that induce post-transcriptional gene silencing (PTGS). Associates mainly with small RNAs of 24 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Targeted by turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO6 function in RNA silencing.

Gene References into Functions

ARGONAUTE 6 bridges transposable element mRNA-derived siRNAs to the establishment of DNA methylation. PMID: 25388951
the identification of the related AGO6 in a forward genetic screen for mutants defective in RNA-directed DNA methylation and transcriptional gene silencing in shoot and root apical meristems in Arabidopsis thaliana PMID: 21998686
AGO6 is important for the accumulation of specific heterochromatin-related siRNAs, and for DNA methylation and transcriptional gene silencing, this function is partly redundant with AGO4. [AGO6] PMID: 17332757

Subcellular Location

Nucleus.

Protein Families

Argonaute family, Ago subfamily

Tissue Specificity

Expressed in roots, cotyledons and shoot meristematic region.

Database Links

KEGG: ath:AT2G32940

STRING: 3702.AT2G32940.1

UniGene: At.53011

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