Recombinant Arabidopsis thaliana Protein disulfide isomerase-like 1-1 (PDIL1-1)

Code CSB-YP893575DOA
MSDS
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Source Yeast
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Code CSB-EP893575DOA
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Source E.coli
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Code CSB-EP893575DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893575DOA
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Source Baculovirus
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Code CSB-MP893575DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PDIL1-1
Uniprot No.
Alternative Names
PDIL1-1; PDI5; At1g21750; F8K7.19; Protein disulfide isomerase-like 1-1; AtPDIL1-1; EC 5.3.4.1; Protein disulfide-isomerase 1; PDI 1; Protein disulfide-isomerase 5; AtPDI5
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
24-501
Target Protein Sequence
EETETKE FVLTLDHTNF TDTINKHDFI VVEFYAPWCG HCKQLAPEYE KAASALSSNV PPVVLAKIDA SEETNREFAT QYEVQGFPTI KIFRNGGKAV QEYNGPREAE GIVTYLKKQS GPASAEIKSA DDASEVVSDK KVVVVGIFPK LSGSEFDSFM AIAEKLRSEL DFAHTSDAKL LPRGESSVTG PVVRLFKPFD EQFVDSKDFD GEALEKFVKE SSIPLITVFD KDPNNHPYVI KFFESTNTKA MLFINFTGEG AESLKSKYRE VATSNKGQGL SFLLGDAENS QGAFQYFGLE ESQVPLIIIQ TADDKKYLKT NVEVDQIESW VKDFKDGKIA PHKKSQPIPA ENNEPVKVVV SDSLDDIVLN SGKNVLLEFY APWCGHCQKL APILDEVAVS YQSDSSVVIA KLDATANDFP KDTFDVKGFP TIYFKSASGN VVVYEGDRTK EDFISFVDKN KDTVGEPKKE EETTEEVKDE L
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein disulfide isomerase that associates with RD21A protease for trafficking from the ER through the Golgi to lytic and protein storage vacuoles of endothelial cells in developing seeds. Regulates the timing of programmed cell death (PCD) of the endothelial cells by chaperoning and inhibiting cysteine proteases during their trafficking to vacuoles.
Gene References into Functions
  1. Data suggest that PDI5 is required for proper seed development and regulates the timing of PCD by chaperoning and inhibiting Cys proteases during their trafficking to vacuoles before PCD of the endothelial cells. [PDI5] PMID: 18676877
Subcellular Location
Endoplasmic reticulum lumen. Vacuole. Note=Found in protein storage vacuoles and lytic vacuoles in endothelial cells of developing seeds.
Protein Families
Protein disulfide isomerase family
Tissue Specificity
Highly expressed in flowers, stems and immature seeds, and at lower levels in leaves and siliques (at protein level).
Database Links

KEGG: ath:AT1G21750

STRING: 3702.AT1G21750.1

UniGene: At.24814

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