Recombinant Arabidopsis thaliana Protein kinase 2A, chloroplastic (APK2A)

Code CSB-YP527420DOA
MSDS
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Source Yeast
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Code CSB-EP527420DOA
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Source E.coli
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Code CSB-EP527420DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP527420DOA
MSDS
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Source Baculovirus
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Code CSB-MP527420DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PBL2
Uniprot No.
Alternative Names
PBL2; APK2A; KIN1; At1g14370; F14L17.14; Probable serine/threonine-protein kinase PBL2; EC 2.7.11.1; PBS1-like protein 2; Protein kinase 2A
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
73-426
Target Protein Sequence
AFTFNELK NATKNFRQDN LLGEGGFGCV FKGWIDQTSL TASRPGSGIV VAVKQLKPEG FQGHKEWLTE VNYLGQLSHP NLVLLVGYCA EGENRLLVYE FMPKGSLENH LFRRGAQPLT WAIRMKVAVG AAKGLTFLHE AKSQVIYRDF KAANILLDAD FNAKLSDFGL AKAGPTGDNT HVSTKVIGTH GYAAPEYVAT GRLTAKSDVY SFGVVLLELI SGRRAMDNSN GGNEYSLVDW ATPYLGDKRK LFRIMDTKLG GQYPQKGAFT AANLALQCLN PDAKLRPKMS EVLVTLEQLE SVAKPGTKHT QMESPRFHHS SVMQKSPVRY SHDRPLLHMT PGASPLPSYT QSPRVR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in disease resistance signaling. Contributes to pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling and defense responses downstream of FLS2. Acts as a BIK1 decoy and enables Xanthomonas campestris AvrAC/XopAC detection; X.campestris effector AvrAC/XopAC-mediated uridylylation promotes the formation of a complex with RKS1 and RPP13L4/ZAR1 which, in turn, activates effector-triggered immunity (ETI) against X.campestris. Promotes, when uridylylated by AvrAC/XopAC, the release of ADP from the inactive RKS1-ZAR1 complex, thus activating the resistosome.
Subcellular Location
Cell membrane; Lipid-anchor. Nucleus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Strongly expressed in leaves, moderately in roots, and barely in flowers, mostly in pedicels.
Database Links

KEGG: ath:AT1G14370

STRING: 3702.AT1G14370.1

UniGene: At.48188

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