Recombinant Arabidopsis thaliana Protein phosphatase 2C 77 (ABI2)

Code CSB-YP516896DOA
MSDS
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Source Yeast
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Code CSB-EP516896DOA
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Source E.coli
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Code CSB-EP516896DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP516896DOA
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Source Baculovirus
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Code CSB-MP516896DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
ABI2; PP2C77; At5g57050; MHM17.19; Protein phosphatase 2C 77; AtPP2C77; EC 3.1.3.16; Protein ABSCISIC ACID-INSENSITIVE 2; Protein phosphatase 2C ABI2; PP2C ABI2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-423
Target Protein Sequence
MDEVSPAVAV PFRPFTDPHA GLRGYCNGES RVTLPESSCS GDGAMKDSSF EINTRQDSLT SSSSAMAGVD ISAGDEINGS DEFDPRSMNQ SEKKVLSRTE SRSLFEFKCV PLYGVTSICG RRPEMEDSVS TIPRFLQVSS SSLLDGRVTN GFNPHLSAHF FGVYDGHGGS QVANYCRERM HLALTEEIVK EKPEFCDGDT WQEKWKKALF NSFMRVDSEI ETVAHAPETV GSTSVVAVVF PTHIFVANCG DSRAVLCRGK TPLALSVDHK PDRDDEAARI EAAGGKVIRW NGARVFGVLA MSRSIGDRYL KPSVIPDPEV TSVRRVKEDD CLILASDGLW DVMTNEEVCD LARKRILLWH KKNAMAGEAL LPAEKRGEGK DPAAMSAAEY LSKMALQKGS KDNISVVVVD LKGIRKFKSK SLN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), high light stress, response to glucose, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as H(2)O(2) and oxidative burst in response to ABA and dehydration. Represses GHR1 and, to some extent, SRK2E/OST1, kinases involved in the regulation of SLAC1-dependent stomatal closure. Controls negatively fibrillin that is involved in mediating ABA-induced photoprotection. May be implicated in ABA content regulation. Involved in acquired thermotolerance of root growth and seedling survival. Required for the Erwinia amylovora harpin-induced (HrpN) drought tolerance. Involved in the hydrotropic response.
Gene References into Functions
  1. The role of ABI2 in the activation of nuclear genes during transcription of chloroplast genes PMID: 25976841
  2. findings suggest that ABI2 may functionally link stress-regulated control of growth and nitrate uptake and utilization, which are energy-expensive processes. PMID: 25943353
  3. AtOZF2 is involved in the abscissic acid and salt stress response through the ABI2-mediated signaling pathway. PMID: 22121246
Protein Families
PP2C family
Database Links

KEGG: ath:AT5G57050

STRING: 3702.AT5G57050.1

UniGene: At.22051

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