Recombinant Arabidopsis thaliana Protein-tyrosine sulfotransferase (TPST), partial

Code CSB-YP665598DOA
MSDS
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Source Yeast
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Code CSB-EP665598DOA
MSDS
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Source E.coli
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Code CSB-EP665598DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP665598DOA
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Source Baculovirus
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Code CSB-MP665598DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TPST
Uniprot No.
Alternative Names
TPST; At1g08030; T6D22.29; Protein-tyrosine sulfotransferase; EC 2.8.2.20; Tyrosylprotein sulfotransferase
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
Gene References into Functions
  1. TPST suppressed ethylene production through the action of peptide hormone phytosulfokine, PSK. [TPST] PMID: 25908239
  2. tyrosylprotein sulfotransferase is expressed throughout the plant body, and the highest levels of expression are in the root apical meristem. [tyrosylprotein sulfotransferase] [TPST] PMID: 19666544
Subcellular Location
Golgi apparatus membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed throughout the plant body, highest levels of expression are in the root apical meristem.
Database Links

KEGG: ath:AT1G08030

STRING: 3702.AT1G08030.1

UniGene: At.47739

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