Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At4g12810 (At4g12810)

Code CSB-YP886778DOA
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Source Yeast
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Code CSB-EP886778DOA
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Source E.coli
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Code CSB-EP886778DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP886778DOA
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Source Baculovirus
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Code CSB-MP886778DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KIB1
Uniprot No.
Alternative Names
KIB1; At4g12810; T20K18.160F-box/kelch-repeat protein KIB1; Protein KINK SUPPRESSED IN BZR1-1D 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-382
Target Protein Sequence
MTHKKQKKEM SDSEKKTFNE DSKHSILAVD LVRLILERLS FVDFHRARCV SSIWYIASKT VIGVTNPTTP WLILFPKGDV EIKKDSCKLY DPHENKTYIV RDLGFDLVTS RCLASSGSWF LMLDHRTEFH LLNLFTRVRI PLPSLESTRG SDIKIGNAVL WVDEQRKDYL VVWNISSLFG YHKKGDDRWK VFKPLENERC IIAMVFKENK LYVLSVDGNV DVFYFSGNDS PVRCATLPSS PLRKGHKVVV TLSGEVLIIV AKVEPYPRTR LCFFAVYKMD PKSSRWETIK SLAGEALILD LGITVEAKVM KNCIYFSNDQ FHRYNENSLW NVSNKSGVFV YHFRSANVVQ LVELLTASSR TSKILFKDAR CFFPTFTSKW LL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Required for brassinosteroid (BR) signal transduction. Mediates ASK7/BIN2/SK21 inactivation both by competing with substrate binding (e.g. BZR1) and by promoting its ubiquitination and subsequent proteasomal degradation.
Gene References into Functions
  1. KIB1 plays an essential role in brassinosteroid signaling by inhibiting BIN2 through dual mechanisms of blocking substrate access and promoting degradation. [KIB1] PMID: 28575660
Subcellular Location
Cytoplasm. Nucleus, nucleolus.
Tissue Specificity
Expressed in seedlings, leaves, stems, flower buds and flowers.
Database Links

KEGG: ath:AT4G12810

UniGene: At.65371

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