Recombinant Arabidopsis thaliana Putative GATA transcription factor 22 (GATA22)

Code CSB-YP886828DOA
MSDS
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Source Yeast
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Code CSB-EP886828DOA
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Source E.coli
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Code CSB-EP886828DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP886828DOA
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Source Baculovirus
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Code CSB-MP886828DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GATA22
Uniprot No.
Alternative Names
GATA22; CGA1; GNL; At4g26150; F20B18.260Putative GATA transcription factor 22; Protein CYTOKININ-RESPONSIVE GATA FACTOR 1; Protein GNC-LIKE; AtGNL
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-352
Target Protein Sequence
MGSNFHYTID LNEDQNHQPF FASLGSSLHH HLQQQQQQQQ HFHHQASSNP SSLMSPSLSY FPFLINSRQD QVYVGYNNNT FHDVLDTHIS QPLETKNFVS DGGSSSSDQM VPKKETRLKL TIKKKDNHQD QTDLPQSPIK DMTGTNSLKW ISSKVRLMKK KKAIITTSDS SKQHTNNDQS SNLSNSERQN GYNNDCVIRI CSDCNTTKTP LWRSGPRGPK SLCNACGIRQ RKARRAAMAT ATATAVSGVS PPVMKKKMQN KNKISNGVYK ILSPLPLKVN TCKRMITLEE TALAEDLETQ SNSTMLSSSD NIYFDDLALL LSKSSAYQQV FPQDEKEAAI LLMALSHGMV HG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters. Involved in the modulation of chloroplast development, growth and division in a cytokinin-dependent manner. Repressor of the gibberellic acid (GA) signaling pathway that regulates flowering and modulates greening, in a SOC1-dependent manner. Prevents the accumulation of SOC1 during flowering. Promotes chlorophyll biosynthesis throughout the plant, by regulating chlorophyll biosynthetic genes (e.g. HEMA1 and GUN4) and chloroplast localized glutamate synthase (e.g. GLU1). Involved in the regulation of sugar-sensing genes (e.g. HXK1, HXK2, STP13 and PLT6). Regulator of germination, senescence, elongation growth and flowering time. Influences also leaf starch content.
Gene References into Functions
  1. Constitutive Cga1 overexpression increases both chloroplast biogenesis and starch production but also results in delayed senescence and reduced grain filling. Cga1 regulates chloroplast development in higher plants. PMID: 23548780
  2. Data indicate that CARBON-METABOLISM INVOLVED (GNC) and GNC-LIKE (GNL)/CYTOKININ-RESPONSIVE GATA FACTOR1 (CGA1) expression is repressed by AUXIN RESPONSE FACTO ARF2 and ARF7. PMID: 23878229
  3. GNC and CGA1 regulate chlorophyll production, and positively regulate chloroplast growth in the hypocotyl. PMID: 22811435
  4. CGA1 encodes a GATA factor, whose expression is rapidly induced by both the light and cytokinin signals in Arabidopsis thaliana.[CGA1] PMID: 17587690
Subcellular Location
Nucleus.
Protein Families
Type IV zinc-finger family, Class B subfamily
Tissue Specificity
Expressed predominantly in leaves, and barely in stems, flowers and siliques.
Database Links

KEGG: ath:AT4G26150

STRING: 3702.AT4G26150.1

UniGene: At.32205

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