Recombinant Arabidopsis thaliana Pyridoxal biosynthesis protein PDX2 (PDX2)

Code CSB-YP811870DOA
MSDS
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Source Yeast
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Code CSB-EP811870DOA
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Source E.coli
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Code CSB-EP811870DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP811870DOA
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Source Baculovirus
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Code CSB-MP811870DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PDX2
Uniprot No.
Alternative Names
PDX2; EMB2407; At5g60540; muf9.190; Probable pyridoxal 5'-phosphate synthase subunit PDX2; AtPDX2; EC 4.3.3.6; Protein EMBRYO DEFECTIVE 2407; Pyridoxal 5'-phosphate synthase glutaminase subunit; EC 3.5.1.2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-255
Target Protein Sequence
MTVGVLALQG SFNEHIAALR RLGVQGVEIR KADQLLTVSS LIIPGGESTT MAKLAEYHNL FPALREFVKM GKPVWGTCAG LIFLADRAVG QKEGGQELVG GLDCTVHRNF FGSQIQSFEA DILVPQLTSQ EGGPETYRGV FIRAPAVLDV GPDVEVLADY PVPSNKVLYS SSTVQIQEED ALPETKVIVA VKQGNLLATA FHPELTADTR WHSYFIKMTK EIEQGASSSS SKTIVSVGET SAGPEPAKPD LPIFQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PDX1. Involved in the indirect resistance to singlet oxygen-generating photosensitizers.
Gene References into Functions
  1. Based on a model of the PDX1/PDX2 complex, mutation of a single residue uncouples enzyme coordination and in turn provides tangible evidence for the existence of the recently proposed ammonia tunnel through the core of PDX1. PMID: 17468224
Subcellular Location
Cytoplasm.
Protein Families
Glutaminase PdxT/SNO family
Tissue Specificity
Strongly expressed in roots, stems, leaves and flowers.
Database Links

KEGG: ath:AT5G60540

STRING: 3702.AT5G60540.1

UniGene: At.29154

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