Recombinant Arabidopsis thaliana Ras-related protein RABF2a (RABF2A)

Code CSB-YP333619DOA
MSDS
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Source Yeast
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Code CSB-EP333619DOA
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Source E.coli
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Code CSB-EP333619DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333619DOA
MSDS
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Source Baculovirus
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Code CSB-MP333619DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RABF2A
Uniprot No.
Alternative Names
RABF2A; RAB5A; RHA1; At5g45130; K17O22.15; Ras-related protein RABF2a; AtRABF2a; Ras-related protein Rab5A; AtRab5A; Ras-related protein Rha1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-200
Target Protein Sequence
MASSGNKNIN AKLVLLGDVG AGKSSLVLRF VKDQFVEFQE STIGAAFFSQ TLAVNDATVK FEIWDTAGQE RYHSLAPMYY RGAAAAIIVF DITNQASFER AKKWVQELQA QGNPNMVMAL AGNKADLLDA RKVSAEEAEI YAQENSLFFM ETSAKTATNV KDIFYEIAKR LPRVQPAENP TGMVLPNGPG ATAVSSSCCA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the trafficking of soluble cargo proteins from the prevacuolar compartment to the central vacuole. Involved in vacuolar transport of storage proteins with EREX as effector. Regulates membrane trafficking to protein storage vacuoles (PSVs).
Gene References into Functions
  1. Rha1 and Ara7 localize to the perivacuolar compartment(PVC) and that GTP-binding motifs as well as the effector domain are important for localization of Rha1 to the PVC PMID: 15509844
  2. Localization to endosomes. PMID: 15546360
  3. The gene, cloned through TAIL-PCR, was found to be a heat-shock factor that maps on chromosome 5, close to and above the RFLP marker m61 and the rha1 structure, mRNA, and translation product are reported. PMID: 18381353
Subcellular Location
Endosome membrane; Lipid-anchor. Prevacuolar compartment membrane; Lipid-anchor.
Protein Families
Small GTPase superfamily, Rab family
Tissue Specificity
High in stem, root, and inflorescence.
Database Links

KEGG: ath:AT5G45130

STRING: 3702.AT5G45130.1

UniGene: At.23631

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