Recombinant Arabidopsis thaliana Receptor protein kinase CLAVATA1 (CLV1), partial

Code CSB-YP866025DOA
MSDS
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Source Yeast
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Code CSB-EP866025DOA
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Source E.coli
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Code CSB-EP866025DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866025DOA
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Source Baculovirus
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Code CSB-MP866025DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLV1
Uniprot No.
Alternative Names
CLV1; At1g75820; T4O12.5Receptor protein kinase CLAVATA1; EC 2.7.11.1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the detection of CLV3 and CLV3-like (CLE) peptides, that act as extracellular signals regulating meristem maintenance. Acts with CLV3p as a ligand-receptor pair in a signal transduction pathway coordinating growth between adjacent meristematic regions and controlling the balance between meristem cell proliferation and differentiation.
Gene References into Functions
  1. Data indicate a role for transmembrane receptor kinase CLV1 (CLV1) separable from other stem cell receptors and provides a framework for dissecting the signaling outputs in stem cell regulation. PMID: 28355208
  2. CLV1 signaling in the rib meristem of the shoot apical meristem represses BAM transcription, and is necessary and sufficient for stem cell regulation. PMID: 25758219
  3. The nitrogen (N)-responsive CLE-CLV1 signaling module is an essential mechanism restrictively controlling the expansion of the lateral root system in N-deficient environments. PMID: 24449877
  4. POL and PLL1 act downstream of CLV signaling to maintain WUS expression PMID: 17079273
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
In a central region of the shoot and in early flower meristems.
Database Links

KEGG: ath:AT1G75820

STRING: 3702.AT1G75820.1

UniGene: At.25465

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