Recombinant Arabidopsis thaliana Regulatory protein NPR6 (NPR6)

Code CSB-YP885513DOA
MSDS
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Source Yeast
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Code CSB-EP885513DOA
MSDS
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Source E.coli
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Code CSB-EP885513DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885513DOA
MSDS
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Source Baculovirus
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Code CSB-MP885513DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NPR6
Uniprot No.
Alternative Names
NPR6; BOP1; At3g57130; F24I3.210; Regulatory protein NPR6; BTB/POZ domain-containing protein NPR6; Protein BLADE-ON-PETIOLE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-467
Target Protein Sequence
MSNTFEESLK SMSLDYLNLL INGQAFSDVT FSVEGRLVHA HRCILAARSL FFRKFFCESD PSQPGAEPAN QTGSGARAAA VGGVIPVNSV GYEVFLLLLQ FLYSGQVSIV PHKHEPRSNC GDRGCWHTHC TAAVDLSLDI LAAARYFGVE QLALLTQKHL TSMVEKASIE DVMKVLIASR KQDMHQLWTT CSYLIAKSGL PQEILAKHLP IELVAKIEEL RLKSSMPLRS LMPHHHDLTS TLDLEDQKIR RMRRALDSSD VELVKLMVMG EGLNLDESLA LIYAVENCSR EVVKALLELG AADVNYPAGP TGKTALHIAA EMVSPDMVAV LLDHHADPNV QTVDGITPLD ILRTLTSDFL FKGAIPGLTH IEPNKLRLCL ELVQSAALVI SREEGNNNSN DNNTMIYPRM KDEHTSGSSL DSRLVYLNLG ATNRDIGDDN SNQREGMNLH HHHHDPSTMY HHHHHHF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Acts redundantly with BOP2. BOP1/2 promote leaf and floral meristem fate and determinacy in a pathway targeting AP1 and AGL24. BOP1/2 act as transcriptional co-regulators through direct interaction with TGA factors, including PAN, a direct regulator of AP1. Controls lateral organ fate through positive regulation of adaxial-abaxial polarity genes ATHB-14/PHB, YAB1/FIL and YAB3, and through positive regulation of LOB domain-containing genes LOB, LBD6/AS2 and LBD36. Promotes and maintains a developmentally determinate state in leaf cells through the negative regulation of JAG, JGL and class I KNOX genes. Is also involved in nectary development, formation of normal abscission zones and suppression of bract formation.
Gene References into Functions
  1. functional characterization and role in mechanisms of defective cell growth and proliferation caused by PeBoW deficiency PMID: 27440937
  2. The conserved protein BOP1 is essential for viability. PMID: 26940494
  3. The BSS1/BOP1 protein complex inhibits the transport of BIL1/BZR1 to the nucleus from the cytosol and negatively regulates brassinosteroid signaling.[BSS1] PMID: 25663622
  4. BOP1/2 activity is required for AS2 activation specifically in the proximal region of the leaf and that BOP1 is a direct upstream regulator of AS2 during leaf development. PMID: 20118228
  5. BOP1 plays a key role in Arabidopsis morphogenesis with the distinctive combinatorial architecture of the BTB/POZ and ankyrin repeat domains [BOP1] PMID: 15564519
  6. important regulator of the growth and development of lateral organs (BOP1) PMID: 15800002
  7. Here, we show that two NPR1-like genes, BLADE-ON-PETIOLE1 (BOP1) and BOP2, function redundantly to control growth asymmetry, an important aspect of patterning in leaves and flowers. PMID: 15805484

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Subcellular Location
Cytoplasm. Nucleus.
Database Links

KEGG: ath:AT3G57130

STRING: 3702.AT3G57130.1

UniGene: At.53964

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