Recombinant Arabidopsis thaliana Respiratory burst oxidase homolog protein C (RBOHC), partial

Code CSB-YP529307DOA
MSDS
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Source Yeast
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Code CSB-EP529307DOA
MSDS
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Source E.coli
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Code CSB-EP529307DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP529307DOA
MSDS
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Source Baculovirus
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Code CSB-MP529307DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RBOHC
Uniprot No.
Alternative Names
RBOHC; RHD2; At5g51060; K3K7.25; Respiratory burst oxidase homolog protein C; EC 1.11.1.-; EC 1.6.3.-; NADPH oxidase RBOHC; AtRBOHC; Protein ROOT HAIR DEFECTIVE 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Calcium-dependent NADPH oxidase that generates superoxide. Required for H(2)O(2) production in response to K(+) deficiency and for the generation of reactive oxygen species (ROS) that regulate cell expansion through the activation of Ca(2+) channels.
Gene References into Functions
  1. Genetic crosses between the cas-c1 mutant and scn1 or rhd2 mutants were performed, and the detailed phenotypic and molecular characterization of the double mutants demonstrates that scn1 mutation is epistatic to cas-c1 and cas-c1 is epistatic to rhd2 mutation, indicating that CAS-C1 acts in early steps of the root hair development process. PMID: 29490083
  2. NADPH oxidase was involved in root hair elongation induced by vanadate. PMID: 24833217
  3. findings demonstrate how a positive feedback mechanism involving RHD2, reactive oxygen species, and Ca2+ can determine root hair cell shape PMID: 18309082
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
RBOH (TC 5.B.1.3) family
Tissue Specificity
In roots, expressed in the epidermis in the proximal regions of the meristem, in the elongation zone, in the differentiation zone and in elongating root hairs.
Database Links

KEGG: ath:AT5G51060

STRING: 3702.AT5G51060.1

UniGene: At.29697

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