Recombinant Arabidopsis thaliana SAL1 phosphatase (SAL1)

Code CSB-YP670362DOA
MSDS
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Source Yeast
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Code CSB-EP670362DOA
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Source E.coli
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Code CSB-EP670362DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP670362DOA
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Source Baculovirus
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Code CSB-MP670362DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SAL1
Uniprot No.
Alternative Names
SAL1; FRY1; At5g63980; MBM17.8SAL1 phosphatase; 3'(2'),5'-bisphosphate nucleotidase 1; EC 3.1.3.7; 3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase 1; DPNPase 1; Inositol polyphosphate 1-phosphatase 1; IPPase 1; Inositol-1,4-bisphosphate 1-phosphatase 1; EC 3.1.3.57; Protein FIERY 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-353
Target Protein Sequence
MAYEKELDAA KKAASLAARL CQKVQKALLQ SDVQSKSDKS PVTVADYGSQ AVVSLVLEKE LSSEPFSLVA EEDSGDLRKD GSQDTLERIT KLVNDTLATE ESFNGSTLST DDLLRAIDCG TSEGGPNGRH WVLDPIDGTK GFLRGDQYAV ALGLLEEGKV VLGVLACPNL PLASIAGNNK NKSSSDEIGC LFFATIGSGT YMQLLDSKSS PVKVQVSSVE NPEEASFFES FEGAHSLHDL SSSIANKLGV KAPPVRIDSQ AKYGALSRGD GAIYLRFPHK GYREKIWDHV AGAIVVTEAG GIVTDAAGKP LDFSKGKYLD LDTGIIVANE KLMPLLLKAV RDSIAEQEKA SAL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. May regulate the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. May play a role in the biosynthesis of sulfate conjugates and RNA processing. Is also able to hydrolyze inositol 1,4-bisphosphate and inositol 1,3,4-trisphosphate. Could be considered as a negative regulator of abscisic acid (ABA)- and stress-responsive genes, through modulating the inositol 1,4,5-trisphosphate (IP3) turnover. Is also involved in salt tolerance. Acts as a suppressor of virus- and transgene-induced silencing.
Gene References into Functions
  1. Data indicate that the redox regulation of 3'-phosphoadenosine 5'-phosphate (PAP) phosphatase SAL1 for activation of chloroplast signaling is conserved in the plant kingdom. PMID: 27432987
  2. These results identified SAL1 as a new component mediating cadmium (Cd) toxicity and established the role of the endoplasmic reticulum stress response in Cd toxicity. PMID: 27044671
  3. this study revealed several new phenotypes caused by disruption of FIERY1, the decrease in glucosinolate content and in accumulation of sulfur and potassium. PMID: 22724014
  4. SAL1 regulates its levels by dephosphorylating 3'-phosphoadenosine 5'-phosphate and accumulates in chloroplasts and mitochondria but not in the cytosol PMID: 22128124
  5. RNA silencing modulated by FRY1 and XRN4 plays an important role in shaping root architecture. PMID: 20807376
  6. SAL1 acts in the chloroplast compartment to dephosphorylate phosphoadenosine phosphosulfate. Mutation of the SAL1 gene led to deregulated LOX activity and jasmonic acid accumulation. PMID: 20053710
  7. The ron1-1 mutant and mutants affected in auxin homeostasis share perturbations in venation patterning, lateral root formation, root hair length, shoot branching, and apical dominance. PMID: 20044451
  8. HOS2 is identical to the FIERY1 gene previously described. PMID: 15500469
  9. FRY1 modifies hypocotyl length in a light-dependent fashion and affects light signaling through its 3'(2')5'-bisphosphate nucleotidase activity. PMID: 19077168
  10. SAL1 acts as a negative regulator of predominantly ABA-independent and also ABA-dependent stress response pathways prolonged water stress. PMID: 19170934

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Protein Families
Inositol monophosphatase superfamily
Tissue Specificity
Expressed in roots, leaves, stems, flowers and siliques.
Database Links

KEGG: ath:AT5G63980

STRING: 3702.AT5G63980.1

UniGene: At.9555

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