Recombinant Arabidopsis thaliana SWI/SNF complex subunit SWI3B (SWI3B)

Code CSB-YP774590DOA
MSDS
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Source Yeast
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Code CSB-EP774590DOA
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Source E.coli
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Code CSB-EP774590DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP774590DOA
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Source Baculovirus
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Code CSB-MP774590DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SWI3B
Uniprot No.
Alternative Names
SWI3B; CHB2; At2g33610; F4P9.38; SWI/SNF complex subunit SWI3B; AtSWI3B; Transcription regulatory protein SWI3B
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-469
Target Protein Sequence
MAMKAPDPGG SGEILPSTPS LSETTSGGAA AASKSAQLPS SSSDIDNIHV PSYSSWFSWT DINDCEVRSL PEFFDSRSSS KNPKFYLYLR NSIIKQYRDD HPRKISFTDV RRTLVSDVVS IRRVFDFLDS WGLINYNSSA SAKPLKWEEK EAGKSAGDAA SEPATTVKET AKRNCNGCKA ICSIACFACD KYDLTLCARC YVRSNYRVGI NSSEFKRVEI SEESKPEWSD KEILLLLEAV MHYGDDWKKV ASHVIGRTEK DCVSQFVKLP FGEQFVKESD SEDGLEMFDQ IKDSDIPESE GIDKDGSSPN KRIKLTPLAD ASNPIMAQAA FLSALAGTNV AEAAARAAVR ALSDVDYEAD KNASRDPNRQ DANAASSGET TRNESERAWA DAKSLIEKEE HEVEGAIKET VEVEMKKIRD RIVHFEKLDL EMERSRKQLE EVRNLLFVDQ LNIFFHTRKA RKTEDRIEC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of a multiprotein complex equivalent of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, which is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. May play an essential role in the transition from the vegetative to the reproductive phase of development. May be a positive regulator of ABA signaling.
Gene References into Functions
  1. regulates IAMT1 expression via chromatin remodeling in leaf development PMID: 29650150
  2. SWI3B, an essential subunit of the SWI/SNF complex, physically interacts with a lncRNA-binding protein, IDN2. PMID: 23246435
  3. SWI3B interacts with HAB1 in the cell nucleus. SWI3B mutants showed a reduced sensitivity to abscisic acid-mediated inhibition of seed germination. PMID: 19033529
  4. Data show that homologues of conserved subunits of SWI/SNF-type complexes, including Snf2-type ATPases and SWI3-type proteins, participate in analogous processes in Arabidopsis. PMID: 19301030
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots, stems, leaves, flowers and siliques.
Database Links

KEGG: ath:AT2G33610

STRING: 3702.AT2G33610.1

UniGene: At.43725

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