Recombinant Arabidopsis thaliana Salt tolerance protein (STO)

Code CSB-YP842595DOA
MSDS
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Source Yeast
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Code CSB-EP842595DOA
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Source E.coli
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Code CSB-EP842595DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842595DOA
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Source Baculovirus
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Code CSB-MP842595DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BBX24
Uniprot No.
Alternative Names
BBX24; STO; At1g06040; T21E18.8; T21E18.9B-box zinc finger protein 24; Salt tolerance protein
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-248
Target Protein Sequence
MKIQCDVCEK APATVICCAD EAALCPQCDI EIHAANKLAS KHQRLHLNSL STKFPRCDIC QEKAAFIFCV EDRALLCRDC DESIHVANSR SANHQRFLAT GIKVALTSTI CSKEIEKNQP EPSNNQQKAN QIPAKSTSQQ QQQPSSATPL PWAVDDFFHF SDIESTDKKG QLDLGAGELD WFSDMGFFGD QINDKALPAA EVPELSVSHL GHVHSYKPMK SNVSHKKPRF ETRYDDDDEE HFIVPDLG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as negative regulator of seedling photomorphogenesis and light-regulated inhibition of hypocotyl elongation. BBX24/STO and BBX25/STH function as transcriptional corepressors of HY5 activity, leading to the down-regulation of BBX22 expression. BBX24/STO acts additively with BBX25/STH during de-etiolation and the hypocotyl shade avoidance response. Functions as negative regulator of photomorphogenic UV-B responses by interacting with both COP1 and HY5. May act as a transcription factor in the salt-stress response.
Gene References into Functions
  1. BBX24 physically interacts with DELLA proteins and alleviates DELLA-mediated repression of PIF4 activity.[BBX24] PMID: 25656233
  2. Data indicate that the expression of the B-box family gene STO (BBX24) is controlled by circadian rhythm and is affected by environmental factors and phytohormones PMID: 24498334
  3. BX24 and BBX25 physically interact with HYH which leads to depletion of HYH molecules from the active pool and, thus indirectly, reduce the function of HY5 in promoting photomorphogenesis. [BBX24] PMID: 23733077
  4. STO/BBX24 functions as a negative regulator of photomorphogenic UV-B responses by interacting with both COP1 and HY5. PMID: 22410790
  5. Microscopic analysis revealed that the STO:eGFP fusion protein is located in the nucleus. PMID: 17605755

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Subcellular Location
Nucleus.
Tissue Specificity
High expression in leaves and lower in roots and flowers.
Database Links

KEGG: ath:AT1G06040

STRING: 3702.AT1G06040.1

UniGene: At.23289

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