Recombinant Arabidopsis thaliana Serine--glyoxylate aminotransferase (AGT1)

Code CSB-YP707120DOA
MSDS
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Source Yeast
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Code CSB-EP707120DOA
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Source E.coli
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Code CSB-EP707120DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP707120DOA
MSDS
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Source Baculovirus
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Code CSB-MP707120DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGT1
Uniprot No.
Alternative Names
AGT1; At2g13360; F14O4.7; Serine--glyoxylate aminotransferase; EC 2.6.1.45; Alanine--glyoxylate aminotransferase; AGT; EC 2.6.1.44; Asparagine aminotransferase; EC 2.6.1.-; Serine--pyruvate aminotransferase; EC 2.6.1.51
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-401
Target Protein Sequence
MDYMYGPGRH HLFVPGPVNI PEPVIRAMNR NNEDYRSPAI PALTKTLLED VKKIFKTTSG TPFLFPTTGT GAWESALTNT LSPGDRIVSF LIGQFSLLWI DQQKRLNFNV DVVESDWGQG ANLQVLASKL SQDENHTIKA ICIVHNETAT GVTNDISAVR TLLDHYKHPA LLLVDGVSSI CALDFRMDEW GVDVALTGSQ KALSLPTGLG IVCASPKALE ATKTSKSLKV FFDWNDYLKF YKLGTYWPYT PSIQLLYGLR AALDLIFEEG LENIIARHAR LGKATRLAVE AWGLKNCTQK EEWISNTVTA VMVPPHIDGS EIVRRAWQRY NLSLGLGLNK VAGKVFRIGH LGNVNELQLL GCLAGVEMIL KDVGYPVVMG SGVAAASTYL QHHIPLIPSR I
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Photorespiratory enzyme that catalyzes transamination reactions with multiple substrates, including asparagine. Functions exclusively as a catabolic enzyme in Asn metabolism. Involved in root development during seedling establishment after seed germination by regulating serine homeostasis and acetate conversion.
Gene References into Functions
  1. the enzyme serine:glyoxylate aminotransferase encoded by AGT1 has been identified as an asparagine aminotransferase in Arabidopsis PMID: 24461228
  2. The results suggest that AGT1 is involved in Asn metabolism in Arabidopsis. PMID: 23098902
Subcellular Location
Peroxisome.
Protein Families
Class-V pyridoxal-phosphate-dependent aminotransferase family
Tissue Specificity
Widely expressed. Preferentially expressed in green, leafy tissues, root cortex and epidermis, developing siliques and dry seeds.
Database Links

KEGG: ath:AT2G13360

STRING: 3702.AT2G13360.1

UniGene: At.24853

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