Recombinant Arabidopsis thaliana Serine/threonine-protein kinase PBS1 (PBS1)

Code CSB-YP880708DOA
MSDS
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Source Yeast
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Code CSB-EP880708DOA
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Source E.coli
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Code CSB-EP880708DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880708DOA
MSDS
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Source Baculovirus
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Code CSB-MP880708DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PBS1
Uniprot No.
Alternative Names
PBS1; At5g13160; T19L5.120; Serine/threonine-protein kinase PBS1; EC 2.7.11.1; AvrPphB susceptible protein 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-456
Target Protein Sequence
MGCFSCFDSS DDEKLNPVDE SNHGQKKQSQ PTVSNNISGL PSGGEKLSSK TNGGSKRELL LPRDGLGQIA AHTFAFRELA AATMNFHPDT FLGEGGFGRV YKGRLDSTGQ VVAVKQLDRN GLQGNREFLV EVLMLSLLHH PNLVNLIGYC ADGDQRLLVY EFMPLGSLED HLHDLPPDKE ALDWNMRMKI AAGAAKGLEF LHDKANPPVI YRDFKSSNIL LDEGFHPKLS DFGLAKLGPT GDKSHVSTRV MGTYGYCAPE YAMTGQLTVK SDVYSFGVVF LELITGRKAI DSEMPHGEQN LVAWARPLFN DRRKFIKLAD PRLKGRFPTR ALYQALAVAS MCIQEQAATR PLIADVVTAL SYLANQAYDP SKDDSRRNRD ERGARLITRN DDGGGSGSKF DLEGSEKEDS PRETARILNR DINRERAVAE AKMWGESLRE KRRQSEQGTS ESNSTG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protein kinase required for plant defense mechanism mediated by the disease resistance (R) protein RPS5. In case of infection by Pseudomonas syringae, AvrPphB triggers RPS5-mediated defense mechanism via the cleavage of PBS1. Both kinase activity and cleavage by avrPphB are independently required to trigger the RPS5-mediated resistance. Contributes to PAMP-triggered immunity (PTI) signaling and defense responses downstream of FLS2.
Gene References into Functions
  1. PBS1 is targeted to the plasma membrane via S-acylation at its N terminus and that plasma membrane localization is required for RPS5 activation. PMID: 24225654
  2. Activation of RPS5 did not require PBS1 kinase function, and thus RPS5 appears to sense a subtle conformational change in PBS1, rather than cleavage. PMID: 22372664
  3. The pathogenic Pseudomonas syringae effector AvrPphB cleaves the Arabidopsis receptor-like cytoplasmic kinase PBS1 to trigger cytoplasmic immune receptor RPS5-specified effector-triggered immunity (ETI). PMID: 20413097
Subcellular Location
Cell membrane; Lipid-anchor.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Database Links

KEGG: ath:AT5G13160

STRING: 3702.AT5G13160.1

UniGene: At.23518

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