Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly
specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant
protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide
linkage between the biotin and the specific lysine of the AviTag.
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