Recombinant Arabidopsis thaliana Signal recognition particle 54 kDa protein, chloroplastic (FFC)

Code CSB-YP334226DOA
MSDS
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Source Yeast
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Code CSB-EP334226DOA
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Source E.coli
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Code CSB-EP334226DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334226DOA
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Source Baculovirus
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Code CSB-MP334226DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FFC
Uniprot No.
Alternative Names
FFC; CPSRP54; At5g03940; F8F6_150; Signal recognition particle 54 kDa protein; chloroplastic; 54 chloroplast protein; 54CP; SRP54; cpSRP54; FFC
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
76-564
Target Protein Sequence
EMFGQ LTGGLEAAWS KLKGEEVLTK DNIAEPMRDI RRALLEADVS LPVVRRFVQS VSDQAVGMGV IRGVKPDQQL VKIVHDELVK LMGGEVSELQ FAKSGPTVIL LAGLQGVGKT TVCAKLACYL KKQGKSCMLI AGDVYRPAAI DQLVILGEQV GVPVYTAGTD VKPADIAKQG LKEAKKNNVD VVIMDTAGRL QIDKGMMDEL KDVKKFLNPT EVLLVVDAMT GQEAAALVTT FNVEIGITGA ILTKLDGDSR GGAALSVKEV SGKPIKLVGR GERMEDLEPF YPDRMAGRIL GMGDVLSFVE KATEVMRQED AEDLQKKIMS AKFDFNDFLK QTRAVAKMGS MTRVLGMIPG MGKVSPAQIR EAEKNLLVME AMIEVMTPEE RERPELLAES PERRKRIAKD SGKTEQQVSA LVAQIFQMRV KMKNLMGVME GGSIPALSGL EDALKAEQKA PPGTARRKRK ADSRKKFVES ASSKPGPRGF GSGN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in cotranslational and post-translational sorting of thylakoid proteins. Binds GTP specifically. Activates the GTPase activity of CPFTSY when bound together. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids.
Gene References into Functions
  1. cbd is a recessive mutant in the gene cpSRP54 encoding the 54 kDa subunit of the chloroplast signal recognition particle. Relative to wild type, expression increased in carotenoid and abscisic acid catabolic genes. PMID: 22791829
  2. cpSRP54 deletion led to a change in light-harvesting complex composition, an increase of PsbS, a decreased photosystem I/II ratio, and, in young leaves, to up-regulation of thylakoid proteases and stromal chaperones, including ClpC PMID: 18633119
Subcellular Location
Plastid, chloroplast stroma.
Protein Families
GTP-binding SRP family, SRP54 subfamily
Tissue Specificity
Most abundant in green shoot tissue and lower levels seen in the roots and etiolated buds.
Database Links

KEGG: ath:AT5G03940

STRING: 3702.AT5G03940.1

UniGene: At.435

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